Cho So-Hyun, Park Hyoung Joon, Lee Ji Hyun, Do Jung-Ah, Heo Seok, Jo Jeong Hwa, Cho Sooyeul
Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju-si, Chungcheongbuk-do 363-700, Republic of Korea.
Advanced Analysis Team, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju-si, Chungcheongbuk-do 363-700, Republic of Korea.
J Pharm Biomed Anal. 2015;111:138-46. doi: 10.1016/j.jpba.2015.03.018. Epub 2015 Mar 28.
Anabolic-androgenic steroids (AASs) have been illegally used in counterfeit drugs to improve the performance of athletes. In addition, AASs have been used for cosmetic purpose by non-athletes. To determine the presence of 26 AASs, an analysis method using ultra-liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. The validated method was applied to 19 counterfeit drugs collected from the Internet and off-line markets during 2014. Nearly 50% (9/19) of the samples contained one of these 26 AASs. In addition, the concentration ranges of the AASs ranged from 0.09 to 119,228.57 mg/kg in the suspected samples. The determined AASs primarily consisted of testosterone and testosterone 17-propionate (26%) followed by boldenone (21%). These results indicate the adulteration of over-the-counter counterfeit drugs, and the continuous monitoring of counterfeit drugs or dubious dietary supplements containing anabolic steroids is warranted.
合成代谢雄激素类固醇（AASs）已被非法用于假药中以提高运动员成绩。此外，非运动员也将AASs用于美容目的。为了测定26种AASs的存在情况，开发并验证了一种使用超高效液相色谱-串联质谱（UHPLC-MS/MS）的分析方法。该验证方法应用于2014年从互联网和线下市场收集的19种假药。近50%（9/19）的样品含有这26种AASs中的一种。此外，在疑似样品中，AASs的浓度范围为0.09至119,228.57 mg/kg。所测定的AASs主要包括睾酮和17-丙酸睾酮（26%），其次是勃地酮（21%）。这些结果表明非处方假药存在掺假情况，因此有必要持续监测含有合成代谢类固醇的假药或可疑膳食补充剂。
