Van Poucke C, Detavernier C, Van Cauwenberghe R, Van Peteghem C
Ghent University, Department of Bio-analysis, Laboratory for Food Analysis, Harelbekestraat 72, B-9000 Gent, Belgium.
Anal Chim Acta. 2007 Mar 14;586(1-2):35-42. doi: 10.1016/j.aca.2006.09.050. Epub 2006 Oct 4.
Nineteen different dietary supplements, ordered through the internet and intercepted by the Belgian pharmaceutical inspection at the post office, were analyzed by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the presence of anabolic steroids. After a methanolic extraction the samples were screened for the presence of 49 compounds. This resulted in almost 60% of the samples being suspected of containing one of these 49 anabolic compounds and being subjected to a confirmatory product ion scan. In all of these suspected samples we were able to confirm at least one anabolic steroid with concentrations between 0.01 and 2.5 mg unit(-1) (unit: one capsule or tablet or for liquids: the prescribed dose). The anabolic steroids that was mostly encountered was testosterone (50%) followed by beta-boldenone (25%). These results once more confirm the dubious reputation of over-the-counter dietary supplements.
通过互联网订购并在邮局被比利时药品检验部门截获的19种不同的膳食补充剂,采用液相色谱 - 串联质谱法(LC-MS/MS)分析其中是否含有合成代谢类固醇。经甲醇提取后,对样品进行49种化合物的筛查。结果发现,近60%的样品疑似含有这49种合成代谢化合物中的一种,并进行了确证性产物离子扫描。在所有这些疑似样品中,我们能够确认至少一种合成代谢类固醇,其浓度在0.01至2.5毫克/单位(单位:一粒胶囊或一片药,或对于液体:规定剂量)之间。最常检测到的合成代谢类固醇是睾酮(50%),其次是β - 宝丹酮(25%)。这些结果再次证实了非处方膳食补充剂的可疑声誉。