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采用液相色谱-串联质谱法测定膳食补充剂中的合成代谢类固醇。

Determination of anabolic steroids in dietary supplements by liquid chromatography-tandem mass spectrometry.

作者信息

Van Poucke C, Detavernier C, Van Cauwenberghe R, Van Peteghem C

机构信息

Ghent University, Department of Bio-analysis, Laboratory for Food Analysis, Harelbekestraat 72, B-9000 Gent, Belgium.

出版信息

Anal Chim Acta. 2007 Mar 14;586(1-2):35-42. doi: 10.1016/j.aca.2006.09.050. Epub 2006 Oct 4.

Abstract

Nineteen different dietary supplements, ordered through the internet and intercepted by the Belgian pharmaceutical inspection at the post office, were analyzed by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the presence of anabolic steroids. After a methanolic extraction the samples were screened for the presence of 49 compounds. This resulted in almost 60% of the samples being suspected of containing one of these 49 anabolic compounds and being subjected to a confirmatory product ion scan. In all of these suspected samples we were able to confirm at least one anabolic steroid with concentrations between 0.01 and 2.5 mg unit(-1) (unit: one capsule or tablet or for liquids: the prescribed dose). The anabolic steroids that was mostly encountered was testosterone (50%) followed by beta-boldenone (25%). These results once more confirm the dubious reputation of over-the-counter dietary supplements.

摘要

通过互联网订购并在邮局被比利时药品检验部门截获的19种不同的膳食补充剂,采用液相色谱 - 串联质谱法(LC-MS/MS)分析其中是否含有合成代谢类固醇。经甲醇提取后,对样品进行49种化合物的筛查。结果发现,近60%的样品疑似含有这49种合成代谢化合物中的一种,并进行了确证性产物离子扫描。在所有这些疑似样品中,我们能够确认至少一种合成代谢类固醇,其浓度在0.01至2.5毫克/单位(单位:一粒胶囊或一片药,或对于液体:规定剂量)之间。最常检测到的合成代谢类固醇是睾酮(50%),其次是β - 宝丹酮(25%)。这些结果再次证实了非处方膳食补充剂的可疑声誉。

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