含有高溶解氢的透析液有助于硫酸吲哚酚从白蛋白上解离。
Dialysate with high dissolved hydrogen facilitates dissociation of indoxyl sulfate from albumin.
作者信息
Tange Yoshihiro, Takesawa Shingo, Yoshitake Shigenori
机构信息
Department of Medical Engineering, School of Health Science, Kyushu University of Health and Welfare Yoshinomachi, Nobeoka, Japan.
出版信息
Nephrourol Mon. 2015 Feb 25;7(2):e26847. doi: 10.5812/numonthly.26847. eCollection 2015 Mar.
BACKGROUND
Protein-bound toxins such as indoxyl sulfate (IS) are not efficiently removed by conventional hemodialysis (HD).
OBJECTIVES
To improve the removal of IS, we performed an in vitro study to evaluate the effects of high dissolved hydrogen on the dissociation of IS from albumin using simulated HD.
MATERIALS AND METHODS
Wasted dialysate from peritoneal dialysis was concentrated a hundred times using extracorporeal ultrafiltration method. Dialysate with high dissolved hydrogen was made by mixing concentrated dialysis solution and electrolyzed-reduced water. The amounts of free fractions of IS were determined by high performance liquid chromatography.
RESULTS
IS was significantly dissociated from albumin using dialysate with high dissolved hydrogen compared with conventional dialysate (P < 0.05).
CONCLUSIONS
Effective removal of IS is expected using a dialysate with high dissolved hydrogen.
背景
诸如硫酸吲哚酚（IS）之类的蛋白结合毒素不能通过传统的血液透析（HD）有效清除。
目的
为了提高IS的清除率，我们进行了一项体外研究，使用模拟血液透析评估高溶解氢对IS从白蛋白解离的影响。
材料与方法
使用体外超滤法将腹膜透析产生的废弃透析液浓缩100倍。通过将浓缩透析液与电解还原水混合制成高溶解氢的透析液。采用高效液相色谱法测定IS的游离部分含量。
结果
与传统透析液相比，使用高溶解氢的透析液时，IS从白蛋白上的解离显著增加（P < 0.05）。
结论
预期使用高溶解氢的透析液可有效清除IS。
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