Division of Nephrology, Endocrinology and Vascular Medicine, Graduate School of Medicine, Tohoku University, Sendai, Japan.
Nephrol Dial Transplant. 2011 Jul;26(7):2112-8. doi: 10.1093/ndt/gfq727. Epub 2010 Dec 30.
Hydrogen (H(2)) reportedly produces an antioxidative effect by quenching cytotoxic oxygen radicals. We studied the biological effects of water with dissolved H(2) on ischemia-induced cardio-renal injury in a rat model of chronic kidney disease (CKD).
Dahl salt-sensitive rats (7 weeks old) were allowed ad libitum drinking of filtered water (FW: dissolved H(2), 0.00 ± 0.00 mg/L) or water with dissolved H(2) produced by electrolysis (EW: dissolved H(2), 0.35 ± 0.03 mg/L) for up to 6 weeks on a 0.5% salt diet. The rats then underwent ischemic reperfusion (I/R) of one kidney and were killed a week later for investigation of the contralateral kidney and the heart.
In the rats given FW, unilateral kidney I/R induced significant increases in plasma monocyte chemoattractant protein-1, methylglyoxal and blood urea nitrogen. Histologically, significant increases were found in glomerular adhesion, cardiac fibrosis, number of ED-1 (CD68)-positive cells and nitrotyrosine staining in the contralateral kidney and the heart. In rats given EW, those findings were significantly ameliorated and there were significant histological differences between rats given FW and those given EW.
Consumption of EW by ad libitum drinking has the potential to ameliorate ischemia-induced cardio-renal injury in CKD model rats. This indicates a novel strategy of applying H(2) produced by water electrolysis technology for the prevention of CKD cardio-renal syndrome.
据报道，氢气（H₂）通过淬灭细胞毒性氧自由基产生抗氧化作用。我们研究了溶解有 H₂ 的水对慢性肾脏病（CKD）大鼠模型中缺血诱导的心肾损伤的生物学效应。
给予达尔盐敏感大鼠（7 周龄）自由饮用过滤水（FW：溶解 H₂，0.00±0.00mg/L）或通过电解产生的溶解有 H₂ 的水（EW：溶解 H₂，0.35±0.03mg/L），持续 6 周给予 0.5%盐饮食。然后，这些大鼠进行单侧肾脏缺血再灌注（I/R），一周后处死以研究对侧肾脏和心脏。
在给予 FW 的大鼠中，单侧肾脏 I/R 导致血浆单核细胞趋化蛋白-1、甲基乙二醛和血尿素氮显著增加。组织学上，对侧肾脏和心脏中发现肾小球黏附、心脏纤维化、ED-1（CD68）阳性细胞数和硝基酪氨酸染色显著增加。在给予 EW 的大鼠中，这些发现明显改善，给予 FW 的大鼠和给予 EW 的大鼠之间存在显著的组织学差异。
自由饮用 EW 具有改善 CKD 模型大鼠缺血诱导的心肾损伤的潜力。这表明应用水电解技术产生的 H₂ 来预防 CKD 心肾综合征是一种新的策略。
