对 Cyrtocarpa procera Kunth 果实提取物药用特性的评估。

Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts.

作者信息

Martinez-Elizalde Karla Stephanie, Jimenez-Estrada Manuel, Flores Cesar Mateo, Hernandez Luis Barbo, Rosas-Lopez Rocio, Duran-Diaz Angel, Nieto-Yañez Oscar J, Barbosa Elizabeth, Rodriguez-Monroy Marco Aurelio, Canales-Martinez Margarita

机构信息

Laboratorio de Farmacognosia, Unidad de Biotecnologia y Prototipos (UBIPRO) Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Av. De los Barrios No.1, Los Reyes Iztacala, Edo. Mex, Tlalnepantla, C.P. 54090, Mexico.

Instituto de Quimica, UNAM, Distrito Federal, Mexico.

出版信息

BMC Complement Altern Med. 2015 Mar 21;15:74. doi: 10.1186/s12906-015-0602-y.

DOI:10.1186/s12906-015-0602-y

PMID:25887560

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4369878/

Abstract

BACKGROUND

The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species.

METHODS

Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected.

RESULTS

The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC = 4 mg/mL) and Vibrio cholera (MIC = 4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50 = 69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50 = 22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid.

CONCLUSIONS

The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.

摘要

背景

生活在普埃布拉州科克斯卡特兰市圣拉斐尔的人们用Procera Cyrtocarpa的果实治疗胃部疾病。本研究调查了该物种果实的抗菌、抗氧化、细胞毒性和抗炎活性。

方法

通过浸渍法获得甲醇提取物。得到甲醇提取物（MeOH1）后，再获得甲醇亚提取物（MeOH2）和己烷（H）。通过纸片扩散法评估MeOH1、MeOH2和H的抗菌活性。通过使甲醇DPPH溶液脱色来评估自由基的淬灭情况。通过人宫颈癌细胞系（CasKi）生长抑制体外测定系统评估MeOH2的细胞毒性活性。通过酶联免疫吸附测定法（ELISA）测定巨噬细胞系（RAW 264.7）上清液中的白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）。通过柱色谱法分离MeOH2亚提取物，收集了73个馏分。

结果

所检测的革兰氏阳性菌和阴性菌对MeOH1和MeOH2敏感；MeOH2对金黄色葡萄球菌（最低抑菌浓度[MIC]=4mg/mL）和霍乱弧菌（MIC=4mg/mL）具有杀菌作用。MeOH2抑制DPPH自由基（半抑制浓度[SC50]=69.7μg/mL），但细胞毒性试验表明，根据美国国立癌症研究所的标准，该提取物无毒（半数致死剂量[LD50]=22.03μg/mL）。处理后，脂多糖刺激的巨噬细胞产生的促炎细胞因子（IL-1β和TNF-α）减少。甲醇提取物含有多种有机酸，如柠檬酸、棕榈酸和α-亚麻酸。