Department of Microbiology, Srimad Andavan Arts and Science College, Tiruchirapalli-620 005, Tamilnadu, India.
Asian Pac J Trop Med. 2011 Apr;4(4):294-300. doi: 10.1016/S1995-7645(11)60089-8. Epub 2011 May 29.
To evaluate the phytochemical and anti-bacterial efficacy of the seed kernel extract of Mangifera indica (M. indica) against the enteropathogen, Shigella dysenteriae (S. dysenteriae), isolated from the diarrhoeal stool specimens.
The preliminary phytochemical screening was performed by the standard methods as described by Harborne. Cold extraction method was employed to extract the bioactive compounds from mango seed kernel. Disc diffusion method was adopted to screen antibacterial activity. Minimum inhibitory concentration (MIC) was evaluated by agar dilution method. The crude extracts were partially purified by thin layer chromatography (TLC) and the fractions were analyzed by high performance thin layer chromatography (HPTLC) to identify the bioactive compounds.
Phytochemical scrutiny of M. indica indicated the presence of phytochemical constituents such as alkaloids, gums, flavanoids, phenols, saponins, steroids, tannins and xanthoproteins. Antibacterial activity was observed in two crude extracts and various fractions viz. hexane, benzene, chloroform, methanol and water. MIC of methanol fraction was found to be (95±11.8) μg/mL. MIC of other fractions ranged from 130-380 μg/mL.
The present study confirmed that each crude extracts and fractions of M. indica have significant antimicrobial activity against the isolated pathogen S. dysenteriae. The antibacterial activity may be due to the phytochemical constituents of the mango seed kernel. The phytochemical tannin could be the reason for its antibacterial activity.
评估芒果（Mangifera indica）种仁提取物的植物化学成分和抗菌功效，以对抗从腹泻粪便标本中分离出的肠道病原体志贺氏痢疾杆菌（Shigella dysenteriae）。
采用 Harborne 描述的标准方法进行初步植物化学筛选。采用冷萃取法从芒果种仁中提取生物活性化合物。采用圆盘扩散法筛选抗菌活性。采用琼脂稀释法评估最小抑菌浓度（MIC）。采用薄层色谱法（TLC）对粗提取物进行部分纯化，并采用高效薄层色谱法（HPTLC）对各馏分进行分析，以鉴定生物活性化合物。
对芒果的植物化学研究表明，存在多种植物化学成分，如生物碱、树胶、类黄酮、酚类、皂苷、甾体、单宁和黄蛋白。两种粗提取物和各种馏分（正己烷、苯、氯仿、甲醇和水）均显示出抗菌活性。甲醇馏分的 MIC 为（95±11.8）μg/mL。其他馏分的 MIC 范围为 130-380μg/mL。
本研究证实，芒果的各粗提取物和馏分均对分离出的病原体志贺氏痢疾杆菌具有显著的抗菌活性。抗菌活性可能归因于芒果种仁的植物化学成分。植物化学单宁可能是其抗菌活性的原因。
