Liu Wei-Juan, Zhang Xing-Mei, Wang Na, Zhou Xiao-Ling, Fu Yu-Cai, Luo Li-Li
Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Road, Shantou, 515041, China.
Laboratory of Cell Senescence, Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041, China.
Eur J Med Res. 2015 Mar 12;20(1):22. doi: 10.1186/s40001-015-0114-8.
Silent information regulator 2 related enzyme 1 (SIRT1) is one of the key factors in the mechanism of calorie restriction (CR) extending lifespan of animals. The aim of the study is to investigate if CR prolongs ovarian lifespan in mice through activating SIRT1 signaling.
In the present study, 21 female C57BL/6 mice were divided into three groups: the control (n = 7), CR (n = 7), and SRT1720 (n = 7) groups. After the 26-week treatment, the number of ovarian follicles at each stage was counted, and Western blot was performed.
The number of surviving follicles in ovaries of the SRT1720 group was less than that of the CR group but more than that of the normal control (NC) group. The number of atretic follicles in the ovaries of the SRT1720 group was similar to that of the CR group but less than that of the NC group. The number of primordial follicles in the ovaries of the SRT1720 group was less than that of the CR group but more than that of the NC group. The numbers of primary follicles, secondary follicles, antral follicles, and corpora lutea in the SRT1720 group were similar to those in the CR group. Western blot analysis showed that the expression of SIRT1, SIRT6, FOXO3a, and NRF1 proteins was upregulated, and p53 was downregulated in both the CR group and the SRT1720 group compared to the control group.
Our results indicate that CR inhibits the activation of primordial follicles and development of follicles at different stages, thus preserving the reserve of follicle pool (at least partly) through activating SIRT1 signaling.
沉默信息调节因子2相关酶1（SIRT1）是卡路里限制（CR）延长动物寿命机制中的关键因素之一。本研究旨在探讨CR是否通过激活SIRT1信号通路来延长小鼠的卵巢寿命。
在本研究中，将21只雌性C57BL/6小鼠分为三组：对照组（n = 7）、CR组（n = 7）和SRT1720组（n = 7）。经过26周的治疗后，对每个阶段的卵巢卵泡数量进行计数，并进行蛋白质印迹分析。
SRT1720组卵巢中存活卵泡的数量少于CR组，但多于正常对照组（NC）。SRT1720组卵巢中闭锁卵泡的数量与CR组相似，但少于NC组。SRT1720组卵巢中原始卵泡的数量少于CR组，但多于NC组。SRT1720组初级卵泡、次级卵泡、窦状卵泡和黄体的数量与CR组相似。蛋白质印迹分析表明，与对照组相比，CR组和SRT1720组中SIRT1、SIRT6、FOXO3a和NRF1蛋白的表达上调，而p53表达下调。
我们的结果表明，CR抑制原始卵泡的激活和不同阶段卵泡的发育，从而通过激活SIRT1信号通路（至少部分地）保留卵泡池储备。
