Candeias Rui, Casado-Amezúa Pilar, Pearson Gareth A, Serrão Ester A, Teixeira Sara
Centre of Marine Sciences, CCMAR-CIMAR, University of the Algarve, Gambelas Campus, 8005-139, Faro, Portugal.
BMC Res Notes. 2015 Mar 8;8:73. doi: 10.1186/s13104-015-1035-x.
Fucus vesiculosus is a brown seaweed dominant on temperate rocky shores of the northern hemisphere and, is typically distributed in the mid-upper intertidal zone. It is an external fertilizer that reproduces sexually, providing an excellent model to address conflicting theories related to mating systems and sexual selection. Microsatellite markers have been reported for several Fucus species, however the genomic libraries from where these markers have been isolated, have originated from two or more species pooled together (F. vesiculosus and F. serratus in one library; F. vesiculosus, F. serratus and Ascophyllum nodosum in a second library), or when the genomic DNA originated from only one species it was from Fucus spiralis. Although these markers cross-amplify F. vesiculosus individuals, the level of polymorphism has been low for relatedness studies.
The microsatellite markers described here were obtained from an enriched genomic library, followed by 454 pyrosequencing. A total of 9 microsatellite markers were tested across 44 individuals from the North of Portugal. The mean number of alleles across loci was 8.7 and the gene diversity 0.67.
The high variability displayed by these microsatellite loci should be useful for paternity analysis, assessing variance of reproductive success and in estimations of genetic variation within and between populations.
墨角藻是一种褐藻，在北半球温带岩石海岸占主导地位，通常分布在潮间带上中部区域。它是一种有性繁殖的外用肥料，为解决与交配系统和性选择相关的相互矛盾的理论提供了一个极佳的模型。已报道了几种墨角藻属物种的微卫星标记，然而，分离出这些标记的基因组文库来自两个或更多物种的混合样本（一个文库中包含墨角藻和锯齿墨角藻；第二个文库中包含墨角藻、锯齿墨角藻和泡叶藻），或者当基因组DNA仅来自一个物种时，该物种为旋卷墨角藻。尽管这些标记能在墨角藻个体间交叉扩增，但用于亲缘关系研究时，其多态性水平较低。
此处描述的微卫星标记是从一个富集基因组文库中获得的，随后进行了454焦磷酸测序。对来自葡萄牙北部的44个个体测试了总共9个微卫星标记。各基因座的平均等位基因数为8.7，基因多样性为0.67。
这些微卫星基因座显示出的高变异性应有助于进行亲权分析、评估繁殖成功率的方差以及估计种群内部和种群之间的遗传变异。
