López Víctor, Gerique Javier, Langa Elisa, Berzosa César, Valero Marta Sofía, Gómez-Rincón Carlota
Facultad de Ciencias de la Salud, Universidad San Jorge, Autovía A-23 Zaragoza-Huesca km. 299, Villanueva de Gállego (Zaragoza), Spain.
Facultad de Ciencias de la Salud, Universidad San Jorge, Autovía A-23 Zaragoza-Huesca km. 299, Villanueva de Gállego (Zaragoza), Spain.
Res Vet Sci. 2015 Jun;100:148-52. doi: 10.1016/j.rvsc.2015.03.033. Epub 2015 Apr 2.
Anisakis simplex is a foodborne pathogen that can produce human infections and allergic reactions due to the high consumption of raw fish. The seeds of Myristica fragans (Myristicaceae), popularly known as nutmeg, are worldwide used as a culinary spice due to its flavour and properties in food preservation. A nutmeg extract was prepared, analyzed, screened for cytotoxicity and tested against Anisakis simplex L3 larvae. In order to detect the biologically active constituents of the extract, myristicin was tested on the larvae. An acetylcholinesterase inhibition bioassay was also carried out to investigate the antihelmintic mechanism of action. Our results demonstrate that nutmeg exerts antihelmintic effects on Anisakis simplex, being myristicin one of the active compounds. The extract induced a high rate of dead anisakis at concentrations between 0.5 and 0.7 mg/ml without being considered cytotoxic; however, an inhibition of acetylcholinesterase was discarded as the molecular mechanism involved in the activity.
简单异尖线虫是一种食源性病原体，由于生鱼的大量食用会导致人类感染和过敏反应。肉豆蔻（肉豆蔻科）的种子，俗称肉豆蔻，因其风味和食品保鲜特性在全球被用作烹饪香料。制备了肉豆蔻提取物，进行了分析、细胞毒性筛选并针对简单异尖线虫L3幼虫进行了测试。为了检测提取物的生物活性成分，对肉豆蔻醚进行了幼虫测试。还进行了乙酰胆碱酯酶抑制生物测定以研究驱虫作用机制。我们的结果表明，肉豆蔻对简单异尖线虫具有驱虫作用，肉豆蔻醚是活性化合物之一。该提取物在浓度为0.5至0.7毫克/毫升时诱导了高比例的异尖线虫死亡，且不被认为具有细胞毒性；然而，乙酰胆碱酯酶的抑制作用被排除为该活性所涉及的分子机制。
