Harusato Akihito, Flannigan Kyle L, Geem Duke, Denning Timothy L
Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, United States.
Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, United States; Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA 30322, United States.
J Immunol Methods. 2015 Jun;421:20-26. doi: 10.1016/j.jim.2015.03.023. Epub 2015 Apr 17.
The microbiota that populates the mammalian intestine consists of hundreds of trillions of bacteria that are separated from underlying immune cells by a single layer of epithelial cells. The intestinal immune system effectively tolerates components of the microbiota that provide benefit to the host while remaining poised to eliminate those that are harmful. Antigen presenting cells, especially macrophages and dendritic cells, play important roles in maintaining intestinal homeostasis via their ability to orchestrate appropriate responses to the microbiota. Paramount to elucidating intestinal macrophage- and dendritic cell-mediated functions is the ability to effectively isolate and identify these cells from a complex cellular environment. In this review, we summarize methodology for the isolation and phenotypic characterization of macrophages and DCs from the mouse intestine and discuss how this may be useful for gaining insight into the mechanisms by which mucosal immune tolerance is maintained.
寄居于哺乳动物肠道的微生物群由数万亿计的细菌组成，这些细菌与下层免疫细胞仅被一层上皮细胞分隔开来。肠道免疫系统有效地耐受对宿主有益的微生物群成分，同时随时准备清除有害成分。抗原呈递细胞，尤其是巨噬细胞和树突状细胞，通过其协调对微生物群作出适当反应的能力，在维持肠道内环境稳定方面发挥着重要作用。有效从复杂的细胞环境中分离和鉴定这些细胞的能力，对于阐明肠道巨噬细胞和树突状细胞介导的功能至关重要。在本综述中，我们总结了从小鼠肠道中分离巨噬细胞和树突状细胞并进行表型特征分析的方法，并讨论了这对于深入了解维持黏膜免疫耐受的机制可能有何帮助。
