Koscsó Balázs, Gowda Kavitha, Schell Todd D, Bogunovic Milena
Department of Microbiology and Immunology, Penn State University College of Medicine and Milton S. Hershey Medical Center, Hershey, PA 17033, USA.
Department of Microbiology and Immunology, Penn State University College of Medicine and Milton S. Hershey Medical Center, Hershey, PA 17033, USA.
J Immunol Methods. 2015 Jun;421:1-13. doi: 10.1016/j.jim.2015.02.013. Epub 2015 Mar 18.
Mononuclear phagocytes are essential for protecting against pathogens breaching the intestinal mucosa and maintaining the integrity of the gastrointestinal tract. The mononuclear phagocyte family of the healthy intestine is represented by a small population of hematopoietic cells including dendritic cells and macrophages. Distinct mononuclear phagocyte subsets strategically accumulate within and below the mucosal epithelium and are distributed in the submucosa and muscularis externa. Shaped by its unique microenvironment, each mononuclear phagocyte subset is developmentally and functionally unique and phenotypically distinct. Here we summarize our recent advances on identifying and purifying various intestinal mononuclear phagocyte subsets by flow cytometry in the context of their developmental properties and location within the intestinal tissue.
单核吞噬细胞对于抵御突破肠道黏膜的病原体以及维持胃肠道的完整性至关重要。健康肠道中的单核吞噬细胞家族由一小部分造血细胞代表，包括树突状细胞和巨噬细胞。不同的单核吞噬细胞亚群策略性地聚集在黏膜上皮内和下方，并分布于黏膜下层和外肌层。由于其独特的微环境塑造，每个单核吞噬细胞亚群在发育和功能上都是独特的，并且在表型上也有所不同。在此，我们总结了我们最近在通过流式细胞术识别和纯化各种肠道单核吞噬细胞亚群方面的进展，这些进展与它们在肠道组织中的发育特性和位置有关。
