Lee Hee Jung, Jo Suzy, Ryu Jeonghyun, Jeong Han-Sol, Lee Guemsan, Ryu Mi Heon, Jung Myeong Ho, Kim Hyungwoo, Kim Byung Joo
Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do 626‑870, Republic of Korea.
Division of Pharmacology, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do 626‑870, Republic of Korea.
Mol Med Rep. 2015 Aug;12(2):2135-9. doi: 10.3892/mmr.2015.3618. Epub 2015 Apr 15.
Schisandra chinensis Turcz. fruit is widely used to treat skin diseases. The aim of this study was to determine the anti-inflammatory effects of the methanol extract of S. chinensis (MESC) on 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) in mice. The effects of MESC on ear thickness and weight, histopathological changes, immune cell filtration and cytokine production were investigated in DNFB-induced CD mice. Topical application of MESC effectively inhibited ear swelling (30 or 300 μg on the left ear, P<0.001; 30 μg on the right ear, P<0.001). MESC also inhibited hyperplasia, spongiosis (100 μg/ear, P<0.05 and 300 μg/ear, P<0.001, respectively) and immune cell infiltration (100 μg/ear, P<0.05; 300 μg/ear, P<0.001) induced by DNFB. In addition, MESC suppressed increases in tumor necrosis factor (TNF)-α levels (100 or 300 μg/ear, P<0.05), interferon (INF)-γ (30 μg/ear, P<0.05; 100 μg/ear, P<0.01; 300 μg/ear, P<0.001), interleukin (IL)-6 (300 μg/ear, P<0.05) and monocyte chemoattractant protein (MCP)-1 (30 μg/ear, P<0.05; 100 μg/ear, P<0.01; 300 μg/ear, P<0.001). These results suggest that the anti-inflammatory effects of MESC are mediated by the reduced production of TNF-α, IFN-γ, IL-6 and MCP-1, and that MESC has potential use for the treatment of inflammatory skin diseases.
五味子果实被广泛用于治疗皮肤病。本研究的目的是确定五味子甲醇提取物（MESC）对1-氟-2,4-二硝基氟苯（DNFB）诱导的小鼠接触性皮炎（CD）的抗炎作用。在DNFB诱导的CD小鼠中研究了MESC对耳厚度和重量、组织病理学变化、免疫细胞浸润和细胞因子产生的影响。局部应用MESC可有效抑制耳部肿胀（左耳30或300μg，P<0.001；右耳30μg，P<0.001）。MESC还抑制了DNFB诱导的增生、海绵形成（分别为100μg/耳，P<0.05和300μg/耳，P<0.001）和免疫细胞浸润（100μg/耳，P<0.05；300μg/耳，P<0.001）。此外，MESC抑制了肿瘤坏死因子（TNF）-α水平的升高（100或300μg/耳，P<0.05）、干扰素（INF）-γ（30μg/耳，P<0.05；100μg/耳，P<0.01；300μg/耳，P<0.001）、白细胞介素（IL）-6（300μg/耳，P<0.05）和单核细胞趋化蛋白（MCP）-1（30μg/耳，P<0.05；100μg/耳，P<0.01；300μg/耳，P<0.001）。这些结果表明，MESC的抗炎作用是通过减少TNF-α、IFN-γ、IL-6和MCP-1的产生来介导的，并且MESC在治疗炎症性皮肤病方面具有潜在用途。
