白藜芦醇对糖尿病大鼠肾脏晚期糖基化终产物受体及氧化应激的依赖性下调作用
Resveratrol-Dependent Down-regulation of Receptor for Advanced Glycation End-products and Oxidative Stress in Kidney of Rats With Diabetes.
作者信息
Moridi Heresh, Karimi Jamshid, Sheikh Nasrin, Goodarzi Mohammad Taghi, Saidijam Massoud, Yadegarazari Reza, Khazaei Mohammad, Khodadadi Iraj, Tavilani Heidar, Piri Hossein, Asadi Soheila, Zarei Sadegh, Rezaei Azam
机构信息
Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
出版信息
Int J Endocrinol Metab. 2015 Apr 20;13(2):e23542. doi: 10.5812/ijem.23542. eCollection 2015 Apr.
BACKGROUND
Millions of people in the world have diabetes mellitus and its prevalence is growing. Oxidative stress, advanced glycation end-products (AGEs) and receptor for advanced glycation end-products (RAGE) play key role in the pathogenesis of diabetes. New and safe strategies of remedy are needed for this disease.
OBJECTIVES
We hypothesized that resveratrol may exert a renal protective effect on diabetic rats.
MATERIALS AND METHODS
Male rats with diabetes were treated with or without resveratrol as 1, 5, 10 mg/kg of body weight for 30 days. The total AGEs and malondialdehyde levels in kidney tissues were determined by spectrofluorimetric method and the insulin level was assayed using ELISA. The total antioxidant capacity contents in kidney and the glucose in plasma were measured by a colorimetric assay. The expression of RAGE was assayed in kidneys of all animals using quantitative PCR.
RESULTS
In resveratrol-treated rats with diabetes, malondialdehyde levels, plasma glucose and expression of RAGE were significantly reduced compared with the untreated group. Moreover, the total antioxidant and insulin levels significantly increased in treated rats. There was no significant difference in the AGEs contents among all the groups.
CONCLUSIONS
These results revealed that resveratrol has beneficial effects on kidney by extenuating the oxidative stress and down-regulation of RAGE expression.
背景
世界上数以百万计的人患有糖尿病，且其患病率正在上升。氧化应激、晚期糖基化终产物（AGEs）和晚期糖基化终产物受体（RAGE）在糖尿病发病机制中起关键作用。这种疾病需要新的安全治疗策略。
目的
我们假设白藜芦醇可能对糖尿病大鼠发挥肾脏保护作用。
材料与方法
将糖尿病雄性大鼠分为两组，分别给予或不给予1、5、10mg/kg体重的白藜芦醇，持续治疗30天。采用荧光分光光度法测定肾组织中总AGEs和丙二醛水平，用ELISA法检测胰岛素水平。采用比色法测定肾脏总抗氧化能力含量和血浆葡萄糖水平。使用定量PCR检测所有动物肾脏中RAGE的表达。
结果
与未治疗组相比，白藜芦醇治疗的糖尿病大鼠丙二醛水平、血糖和RAGE表达显著降低。此外，治疗组大鼠的总抗氧化剂和胰岛素水平显著升高。所有组之间的AGEs含量无显著差异。
结论
这些结果表明，白藜芦醇通过减轻氧化应激和下调RAGE表达对肾脏具有有益作用。
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