单壁碳纳米管的遗传毒性：对人类胚胎成纤维细胞的体外研究

Genotoxicity of single-walled carbon nanotubes: in vitro study on human embryonic fibroblast cells.

作者信息

Nikitina V A, Chausheva A I, Suetina I A, Katosova L D, Zheglo D G, Mezentseva M V, Platonova V I, Revazova Yu A, Kutsev S I

机构信息

Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia,

出版信息

Bull Exp Biol Med. 2015 Apr;158(6):812-5. doi: 10.1007/s10517-015-2868-y.

DOI:10.1007/s10517-015-2868-y

PMID:25894783

Abstract

The effects of single-walled carbon nanotubes on the levels of DNA aberrations, chromosome and genome disorders were studied on human embryonic fibroblasts, their karyotype was analyzed by the spectral karyotyping method. The level of DNA aberrations increased after 3-h exposure to the nanotubes. No appreciable increase in the incidence of aberrant metaphases, micronuclei, and chromosome 1, 6, 8, 11, X, and Y aneuploidy after 24- and 48-h incubation with the nanotubes were detected.

摘要

研究了单壁碳纳米管对人类胚胎成纤维细胞DNA畸变、染色体和基因组紊乱水平的影响，采用光谱核型分析方法分析其核型。暴露于纳米管3小时后，DNA畸变水平升高。在与纳米管孵育24小时和48小时后，未检测到异常中期、微核以及1号、6号、8号、11号、X和Y染色体非整倍体发生率有明显增加。