[对从滨海边疆区长角血蜱分离出的波瓦桑病毒（POWV）以及两株蜱传脑炎病毒（TBEV）（黄病毒科，黄病毒属）进行基因特征分析：从哈萨克斯坦全沟硬蜱分离出的阿尔马-阿拉桑病毒（AAV）以及从哈巴罗夫斯克边疆区骚扰伊蚊分离出的马利舍沃病毒]
[Genetic characterisation of Powassan virus (POWV) isolated from Haemophysalis longicornis ticks in Primorye and two strains of Tick-borne encephalitis virus (TBEV) (Flaviviridae, Flavivirus): Alma-Arasan virus (AAV) isolated from Ixodes persulcatus ticks in Kazakhstan and Malyshevo virus isolated from Aedes vexans nipponii mosquitoes in Khabarovsk kray].
作者信息
L'vov D K, Al'khovskiĭ S V, Shchelkanov M Iu, Deriabin P G, Gitel'man A K, Botikov A G, Aristova V A
出版信息
Vopr Virusol. 2014;59(5):18-22.
The complete genomes of the three tick-borne flaviviruses (genus Flavivirus, fam. Bunyaviridae) were sequenced: Povassan virus (POWV, strain LEIV-3070Prm, isolated from Haemophysalis logicornis in Primorsky Krai, Russia in 1977), Alma-Arasan virus (AAV, strain LEIV-1380Kaz, isolated from Ixodes persulcatus ticks in Kazakhstan in 1977) and Malyshevo virus (isolated from a pool of Aedes vexans nipponii mosquitoes, in the Khabarovsk Krai, Russia in 1978). It is shown that AAV and Malyshevo virus are the strains of Tick-borne encephalitis virus (TBEV) and belong to Sibirian and Far-Eastern genotypes, respectively (GenBank ID: AAV KJ744033; strain Malyshevo KJ744034). Phylogenetically AAV is closest related (94,6% nt and 98,3% aa identity) to TBEV strains, isolated in Sibiria (Vasilchenko, Aino, Chita-653, Irkutsk-12). Malyshevo virus is closest related (96,4% nt and 98,3% nt identity) to strains of TBEV, isolated in Far Eastern part of Russia (1230, Spassk-72, Primorye-89). POWV LEIV-3070Prm has 99.7% identity with the prototype strain POWV LB, isolated in Canada and 99.5% of isolates with Far-Eastern strains of POWV (Spassk-9 and Nadezdinsk-1991).
对三种蜱传黄病毒（黄病毒属，布尼亚病毒科）的全基因组进行了测序：波瓦桑病毒（POWV，LEIV - 3070Prm株，1977年从俄罗斯滨海边疆区的嗜群血蜱中分离）、阿尔马 - 阿拉桑病毒（AAV，LEIV - 1380Kaz株，1977年从哈萨克斯坦的全沟硬蜱中分离）和马利舍沃病毒（1978年从俄罗斯哈巴罗夫斯克边疆区的骚扰阿蚊日本亚种蚊群中分离）。结果表明，AAV和马利舍沃病毒是蜱传脑炎病毒（TBEV）的毒株，分别属于西伯利亚和远东基因型（GenBank ID：AAV KJ744033；马利舍沃毒株KJ744034）。在系统发育上，AAV与在西伯利亚分离的TBEV毒株（瓦西里琴科、艾诺、赤塔 - 653、伊尔库茨克 - 12）关系最密切（核苷酸同一性为94.6%，氨基酸同一性为98.3%）。马利舍沃病毒与在俄罗斯远东地区分离的TBEV毒株（1230、斯帕斯克 - 72、滨海边疆区 - 89）关系最密切（核苷酸同一性为96.4%，氨基酸同一性为98.3%）。POWV LEIV - 3070Prm与在加拿大分离的POWV原型株LB的同一性为99.7%，与远东地区的POWV毒株（斯帕斯克 - 9和纳杰日丁斯克 - 1991）的同一性为99.5%。
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