Leonova G N, Belikov S I
Somov Institute of Epidemiology and Microbiology, Vladivostok, 690087, Russia.
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk, 664033, Russia.
Vopr Virusol. 2019;64(5):250-256. doi: 10.36233/0507-4088-2019-64-5-250-256.
To date, a lot of data on molecular genetic characteristics of different tick-borne encephalitis virus strains has appeared. Only on the basis of the E protein genome, sequences of about 1,500 TBEV strains were registered in GenBank.
The purpose of the work - revision and comparative analysis of data on complete genomes sequences of the Far Eastern subtype of TBE virus strains distributed in the Asian part of Eurasian continent.
The data on the complete genomes of 84 strains of TBEV isolated in Asia were used; phylogenetic analysis was performed.
it was shown that variants of the TBEV of the Far Eastern subtype are circulating here and form three separate clusters (Sofjin, Senzhang- и Shkotovo-like strains). Sofjin strain (Sofjin-1953, Sofjin-Chumakov, Sofjin-KSY) was considered to be the reference for Far Eastern TBE virus subtype strains and a cluster of Sofjin-like strains. Sofjin-like strains were not found in China and Japan, but widely distributed throughout the area of Primorsky and Khabarovsk krai. The group of Senzhang-like strains was distributed in China, Eastern Siberia, Khabarovsk krai and northern Primorsky krai, but was not found in Japan (Hokkaido). According to molecular genetic characteristics the youngest and more genetically homogeneous group was the Shkotovo-like strains, isolated in the southern part of Primorsky krai, however not found on Hokkaido Island (Japan).
revision of the complete genome characteristics of TBEV strains revealed the features of micro-evolutionary process of viral populations in the Asian part of Eurasia, show the individual affection of strains to certain territories, as well as detect random finds of such strains in the territories of other natural foci.
迄今为止，已经出现了许多关于不同蜱传脑炎病毒株分子遗传特征的数据。仅基于E蛋白基因组，在GenBank中登记了约1500株蜱传脑炎病毒株的序列。
本研究的目的是对分布在欧亚大陆亚洲部分的蜱传脑炎病毒远东亚型完整基因组序列的数据进行修订和比较分析。
使用了在亚洲分离的84株蜱传脑炎病毒完整基因组的数据；进行了系统发育分析。
结果表明，远东亚型的蜱传脑炎病毒变体在此处传播，并形成三个独立的簇（索夫金、森张和什科托沃样毒株）。索夫金毒株（索夫金-1953、索夫金-丘马科夫、索夫金-KSY）被认为是远东蜱传脑炎病毒亚型毒株和索夫金样毒株簇的参考毒株。在中国和日本未发现索夫金样毒株，但在滨海边疆区和哈巴罗夫斯克边疆区广泛分布。森张样毒株组分布在中国、东西伯利亚、哈巴罗夫斯克边疆区和滨海边疆区北部，但在日本（北海道）未发现。根据分子遗传特征，最年轻且遗传上更同质的组是什科托沃样毒株，它们在滨海边疆区南部分离得到，但在北海道岛（日本）未发现。
对蜱传脑炎病毒株完整基因组特征的修订揭示了欧亚大陆亚洲部分病毒种群的微进化过程特征，显示了毒株对某些地区的个别影响，以及在其他自然疫源地随机发现此类毒株的情况。
