从蚊子和蜱虫中分离出的蜱传脑炎病毒株基因组的比较分析。

Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks.

作者信息

Pukhovskaya N M, Morozova Olga V, Belozerova N B, Bakhmetyeva S V, Vysochina N P, Zdanovskaya N I, Ivanov L I

出版信息

Vopr Virusol. 2017;62(1):30-5.

Abstract

The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier.

摘要

蜱传脑炎病毒（TBEV）拉佐MP36株于2014年8月从哈巴罗夫斯克边疆区拉佐地区采集的骚扰伊蚊群体中分离得到。对拉佐MP36株全基因组（GenBank登录号KT001073）进行系统发育分析，结果显示其与TBEV远东亚型相符，且与以下毒株存在差异：1）来自全沟硬蜱（Ixodes persulcatus）P. Schulze，1930[疫苗株205（JX498939）以及2012 - 2013年分离的赫克齐尔1230株（KF880805）、奇恰戈夫卡株（KP844724）、比罗比詹1354株（KF880805）]；2）来自蚊子[1978年从哈巴罗夫斯克边疆区的骚扰伊蚊日本亚种中分离的马利舍沃株（KJ744034）；2011年从蚊子群体中分离的萨哈林6 - 11株（KF826916）]；3）来自人脑[疫苗株索夫金（JN229223）、格鲁比诺/2004（DQ862460）、卡瓦列罗沃（DQ862460）、斯韦特洛戈里耶（DQ862460）]。从蚊子中分离的3株TBEV毒株（拉佐MP36、马利舍沃和萨哈林6 - 11）进入细胞所需的融合肽对于蜱传黄病毒具有典型结构98 - DRGWGNHCGLFGKGSI - 113。未发现蚊子传播的黄病毒典型的氨基酸转变H104G。来自蚊子的TBEV毒株5' - 和3' - 非翻译（UTR）区域的结构与所有亚型的TBEV毒株同源性为85% - 98%，未发现与俄罗斯远东地区发现的蚊子传播的黄病毒发生重组。所有TBEV分离株的5' - 和3' - UTR二级结构以及a型和B型环化序列（CS）高度同源，与生物宿主和传播媒介无关。来自蚊子、蜱和患者的TBEV分离株基因组的相似性以及这些分离株对新生实验室小鼠和组织培养物的致病性可能表明蚊子在自然疫源地TBEV循环中作为偶然或额外病毒载体可能发挥的作用。