Kumari Vibha, Pandey A K, Chauhan U K, Lakra W S
J Environ Biol. 2015 Mar;36(2):357-61.
Ammonia-oxidizing bacteria (AOB) were isolated from sediment samples of fishponds with an aim to use them for application in biological nitrification of water. Isolation of AOB was done in an inorganic medium and nitrite-producing bacterial isolates were selected. These isolates were further screened by polymerase chain reaction using specific primers forAOB. Out of 119 nitrate positive isolates, only 12 showed positive amplification and yielded a PCR product of ~465 bp. Treatment of aquaculture pond and riverwaterwith one of the bacterial isolate (HC-5) resulted in lowering of soluble ammonia level from 3.50 to 0.05 mgl(-1) and 7.5 to 0.01 mgl(-1), respectively. Partial 16S rRNA gene sequencing of isolate HC-5 identified the microorganism as Pseudomonasstutzeri.
从鱼塘沉积物样本中分离出氨氧化细菌（AOB），目的是将其用于水的生物硝化作用。AOB的分离在无机培养基中进行，并选择了产生亚硝酸盐的细菌分离株。使用针对AOB的特异性引物通过聚合酶链反应对这些分离株进行进一步筛选。在119个硝酸盐阳性分离株中，只有12个显示出阳性扩增，并产生了约465 bp的PCR产物。用其中一种细菌分离株（HC-5）处理水产养殖池塘水和河水后，可溶性氨水平分别从3.50降至0.05 mg l(-1)和从7.5降至0.01 mg l(-1)。分离株HC-5的部分16S rRNA基因测序确定该微生物为施氏假单胞菌。
