Walunjkar Babasaheb C, Parihar Akarsh, Singh Nirbhay Kumar, Parmar L D
J Environ Biol. 2015 Mar;36(2):461-6.
Eight wild and four cultivated pigeonpea genotypes were subjected to RAPD and microsatellite analysis, with 40 primers each. Out of these, eight RAPD and five SSR primers were found polymorphic. RAPD primers showed 100% polymorphism and produced a total of 517 DNA fragments, whereas SSR primers produced 67 fragments and they too showed 100% polymorphism. The RAPD markers revealed highest similarity co-efficient of 0.93 (GT-100 and ICPL-87), whereas the highest similarity co-efficient obtained with SSR markers was 1.00 (GTH-1 and GT-100). Average PIC value obtained with RAPD and SSR were 0.90 and 0.18, respectively. The arithmetic mean heterozygosity and marker index were 0.90 and 22.47 respectively with RAPD marker, whereas the corresponding values for SSR markers were 0.18 and 33.66. Moreover; the four wild genotypes (Cajanus scarabaeoides, Rhyncosia rufescence, Cajanus cajanifolius and Rhyncosia canna) and the four cultivars (GTH-1, GT-100, ICPL-87 and GT-1) grouped distinctly in the same subgroups of the dendrograms obtained with both RAPD and SSR analysis. Therefore, the findings of SSR supplement and validate the results obtained with RAPD analysis.
对8个野生木豆基因型和4个栽培木豆基因型进行了RAPD和微卫星分析，每种分析使用40个引物。其中，发现8个RAPD引物和5个SSR引物具有多态性。RAPD引物显示出100%的多态性，共产生517个DNA片段，而SSR引物产生67个片段，它们也显示出100%的多态性。RAPD标记显示出最高的相似系数为0.93（GT - 100和ICPL - 87），而SSR标记获得的最高相似系数为1.00（GTH - 1和GT - 100）。RAPD和SSR获得的平均PIC值分别为0.90和0.18。RAPD标记的算术平均杂合度和标记指数分别为0.90和22.47，而SSR标记的相应值为0.18和33.66。此外，4个野生基因型（Cajanus scarabaeoides、Rhyncosia rufescence、Cajanus cajanifolius和Rhyncosia canna）和4个栽培品种（GTH - 1、GT - 100、ICPL - 87和GT - 1）在用RAPD和SSR分析获得的树状图的同一亚组中明显聚类。因此，SSR的研究结果补充并验证了RAPD分析获得的结果。
