金纳米棒通过刺激先天免疫反应来抑制呼吸道合胞病毒。
Gold nanorods inhibit respiratory syncytial virus by stimulating the innate immune response.
作者信息
Bawage Swapnil S, Tiwari Pooja M, Singh Ankur, Dixit Saurabh, Pillai Shreekumar R, Dennis Vida A, Singh Shree R
机构信息
Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL, USA.
College of Medicine, University of South Alabama, Mobile, AL, USA.
出版信息
Nanomedicine. 2016 Nov;12(8):2299-2310. doi: 10.1016/j.nano.2016.06.006. Epub 2016 Jul 2.
Respiratory syncytial virus (RSV) causes severe pneumonia and bronchiolitis in infants, children and older adults. The use of metallic nanoparticles as potential therapeutics is being explored against respiratory viruses like Influenza, Parainfluenza and Adenovirus. In this study, we showed that gold nanorods (GNRs) inhibit RSV in HEp-2 cells and BALB/c mice by 82% and 56%, respectively. The RSV inhibition correlated with marked upregulated antiviral genes due to GNR mediated TLR, NOD-like receptor and RIG-I-like receptor signaling pathways. Transmission electron microscopy of lungs showed GNRs in the endocytotic vesicles and histological analyses indicated infiltration by neutrophils, eosinophils and monocytes correlating with clearance of RSV. In addition, production of cytokines and chemokines in the lungs indicates recruitment of immune cells to counter RSV replication. To our knowledge, this is the first in vitro and in vivo report that provides possible antiviral mechanisms of GNRs against RSV.
呼吸道合胞病毒（RSV）可导致婴儿、儿童及老年人患上严重的肺炎和支气管炎。人们正在探索使用金属纳米颗粒作为针对流感、副流感和腺病毒等呼吸道病毒的潜在治疗手段。在本研究中，我们发现金纳米棒（GNRs）在HEp-2细胞和BALB/c小鼠中分别对呼吸道合胞病毒有82%和56%的抑制作用。呼吸道合胞病毒的抑制作用与由于金纳米棒介导的Toll样受体、NOD样受体和视黄酸诱导基因I样受体信号通路导致的抗病毒基因显著上调相关。肺部的透射电子显微镜检查显示内吞小泡中有金纳米棒，组织学分析表明中性粒细胞、嗜酸性粒细胞和单核细胞浸润，这与呼吸道合胞病毒的清除相关。此外，肺部细胞因子和趋化因子的产生表明免疫细胞被募集以对抗呼吸道合胞病毒的复制。据我们所知，这是第一份提供金纳米棒针对呼吸道合胞病毒可能的抗病毒机制的体外和体内报告。
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