Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
J Nat Med. 2013 Apr;67(2):339-49. doi: 10.1007/s11418-012-0691-6. Epub 2012 Jul 28.
Notoginsenosides R1, R4, Fa, and K (N-R1, N-R4, N-Fa, and N-K), as well as ginsenosides Rg1, Rb1, Rd, Re, Rf, Rg2 and Rh1 (G-Rg1, G-Rb1, G-Rd, G-Re, G-Rf, G-Rg2 and G-Rh1) in 47 Notoginseng samples including 1-, 2- and 3-year-old main roots, rhizomes and fibrous roots of Panax notoginseng were determined by high-performance liquid chromatography-diode array detection method. Total contents (%) of the 11 saponins were 9.82-14.57 for 2-year old and 14.20-16.00 for 3-year-old rhizomes; 2.72-4.50 for 2-year-old and 1.98-4.92 for 3-year-old fibrous roots; 1.75-3.05 for 1-year-old whole roots; and 3.71-8.98 for 2-year-old and 7.03-11.23 for 3-year-old main roots. Contents of most saponins and total content of 11 saponins were in the order 3- >2- >1-year-old main root samples. G-Rf content, sum of G-Rf and G-Rh1 were, respectively, 0.08-0.18 and 0.14-0.32 for 2- or 3-year-old rhizomes, and 0.01-0.07 and 0.03-0.10 for 2- or 3-year-old main roots. Combined contents of N-R1, G-Rg1 and G-Rb1 were 5.78-9.37 in 3-year-old main roots, and 2.99-7.13 in 2-year-old main roots, of which nearly one-third of samples were lower than the limit (5 %) in the Chinese Pharmacopoeia. Those of 2- or 3-year-old fibrous roots (1.47-3.83) and 1-year-old whole roots (1.41-2.44) were much lower than the limit, and were considered not suitable for use as Notoginseng. Two-year-old main roots are not appropriate for collection as Notoginseng. Different parts and growth years of P. notoginseng can be identified from each another according to differences in saponin content.
采用高效液相色谱-二极管阵列检测法测定了 47 份三七样品中的 11 种人参皂苷,包括 1 年、2 年和 3 年生主根、根茎和纤维根的 Notoginsenosides R1、R4、Fa 和 K(N-R1、N-R4、N-Fa 和 N-K)以及 Ginsenosides Rg1、Rb1、Rd、Re、Rf、Rg2 和 Rh1(G-Rg1、G-Rb1、G-Rd、G-Re、G-Rf、G-Rg2 和 G-Rh1)。2 年生和 3 年生根茎的 11 种人参皂苷总含量(%)分别为 14.57%和 16.00%;2 年生和 3 年生纤维根的含量分别为 1.98%和 4.92%;1 年生全根的含量为 1.75%;2 年生和 3 年生主根的含量分别为 8.98%和 7.03%。大多数皂苷的含量和 11 种皂苷的总含量均为 3->2->1 年生主根样品。2 年生和 3 年生根茎中 G-Rf 含量、G-Rf 和 G-Rh1 之和分别为 0.08-0.18%和 0.14-0.32%,2 年生和 3 年生主根中分别为 0.01-0.07%和 0.03-0.10%。3 年生主根中 N-R1、G-Rg1 和 G-Rb1 的合并含量为 5.78-9.37%,2 年生主根中为 2.99-7.13%,其中近三分之一的样品含量低于中国药典规定的 5%限量。2 年生和 3 年生纤维根(1.47-3.83%)和 1 年生全根(1.41-2.44%)的含量均低于限量,认为不适合作为三七使用。2 年生主根不宜作为三七采集。根据皂苷含量的差异,可以从不同部位和生长年限的三七中识别出来。