Ning Kang, Huai Hao, Li Mengzhi, Xu Yuli, Wei Fugang, Chen Zhongjian, Wang Yong, Huang Pengcheng, Yu Yuqi, Chen Shilin, Dong Linlin
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China.
College of Biological & Pharmaceutical Sciences, China Three Gorges University, 443002, Yichang, China.
Heliyon. 2024 Sep 10;10(18):e37532. doi: 10.1016/j.heliyon.2024.e37532. eCollection 2024 Sep 30.
is a traditional Chinese medicine rich in many pharmacological components. The root of the 'Miaoxiang Sanqi No. 2' is yellow or greenish yellow, while a novel cultivar-'Wenyuan Ziqi No. 1' shows purple root and is thought to have high medicinal value. Little information is available about the anthocyanin biosynthesis in . root. In this study, we compared the 'Miaoxiang Sanqi No. 2' and 'Wenyuan Ziqi No. 1' in morphological, transcriptional and metabolic levels. The results showed that purple rich in the periderm, rhizome and phloem around cambium of the 'Wenyuan Ziqi No. 1' root and cyanidin 3-O-galactoside was the main anthocyanin causing purple. Moreover, 'Wenyuan Ziqi No. 1' highly accumulated in 155 metabolites, including flavones, phenylpropanoids and lipids. Transcriptome data showed that phenylpropanoid biosynthesis pathway genes are highly expressed in 'Wenyuan Ziqi No. 1'. Conjoint analysis showed that anthocyanin biosynthesis pathway substances were highly accumulated in 'Wenyuan Ziqi No. 1', and the expression level of structural genes involved in anthocyanin biosynthesis pathway was higher in 'Wenyuan Ziqi No. 1'. Meanwhile, eight genes that might be involved in anthocyanin biosynthesis were identified. The comprehensive analysis of two cultivars provides new insights into the understanding of root coloration in .
是一种富含多种药理成分的中药。“妙香三七2号”的根为黄色或黄绿色，而一个新品种——“文渊紫奇1号”的根呈紫色，被认为具有很高的药用价值。关于三七根中花青素生物合成的信息很少。在本研究中，我们在形态、转录和代谢水平上对“妙香三七2号”和“文渊紫奇1号”进行了比较。结果表明，“文渊紫奇1号”根的周皮、根茎和形成层周围韧皮部富含紫色，矢车菊素3-O-半乳糖苷是导致紫色的主要花青素。此外，“文渊紫奇1号”在155种代谢产物中高度积累，包括黄酮类、苯丙烷类和脂质。转录组数据表明，苯丙烷生物合成途径基因在“文渊紫奇1号”中高表达。联合分析表明，花青素生物合成途径物质在“文渊紫奇1号”中高度积累，参与花青素生物合成途径的结构基因在“文渊紫奇1号”中的表达水平更高。同时，鉴定出8个可能参与花青素生物合成的基因。对两个品种的综合分析为理解三七根的着色提供了新的见解。
