Sato Takuma, Mori Shiro, Sakamoto Maya, Arai Yoichi, Kodama Tetsuya
Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo-machi, Aoba, Sendai, Miyagi, 980-8575, Japan; Department of Urology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba, Sendai, Miyagi, 980-8575, Japan.
Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo-machi, Aoba, Sendai, Miyagi, 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba, Sendai, Miyagi, 980-8575, Japan.
PLoS One. 2015 Apr 21;10(4):e0123619. doi: 10.1371/journal.pone.0123619. eCollection 2015.
Direct injection of an anticancer agent into a metastatic lymph node (LN) has not been used as a standard treatment because evidence concerning the efficacy of local administration of a drug into a metastatic LN has not been established. Here we show that the combination of intralymphatic drug delivery with nano/microbubbles (NMBs) and ultrasound has the potential to improve the chemotherapeutic effect. We delivered cis-diamminedichloroplatinum (II) (CDDP) into breast carcinoma cells in vitro and found that apoptotic processes were involved in the antitumor action. Next, we investigated the antitumor effect of intralymphatic chemotherapy with NMBs and ultrasound in an experimental model of LN metastasis using MXH10/Mo-lpr/lpr mice exhibiting lymphadenopathy. The combination of intralymphatic chemotherapy with NMBs and ultrasound has the potential to improve the delivery of CDDP into target LNs without damage to the surrounding normal tissues. The present study indicates that intralymphatic drug delivery with NMBs and ultrasound will potentially be of great benefit in the clinical setting.
将抗癌剂直接注射到转移性淋巴结（LN）中尚未作为标准治疗方法使用，因为关于将药物局部给药到转移性淋巴结中的疗效证据尚未确立。在此，我们表明，纳米/微泡（NMBs）介导的淋巴管内给药与超声相结合有可能提高化疗效果。我们在体外将顺二氯二氨铂（II）（CDDP）递送至乳腺癌细胞，并发现凋亡过程参与了抗肿瘤作用。接下来，我们在使用表现出淋巴结病的MXH10/Mo-lpr/lpr小鼠的LN转移实验模型中研究了NMBs介导的淋巴管内化疗与超声的抗肿瘤效果。NMBs介导的淋巴管内化疗与超声相结合有可能在不损害周围正常组织的情况下提高CDDP向靶淋巴结的递送。本研究表明，NMBs介导的淋巴管内给药与超声在临床环境中可能具有巨大益处。
