Ikeda M, Morgenstern C
Department of Otolaryngology, Nihon University School of Medicine, Tokyo, Japan.
Ann Otol Rhinol Laryngol. 1989 Dec;98(12 Pt 1):975-9. doi: 10.1177/000348948909801211.
Twenty guinea pigs were immunized with horseradish peroxidase (HRP) intradermally and challenged with 5 mg of the same antigen in the tympanic bulla. The appearance of immunoglobulin-producing cells (plasma cells) in the inner ear structure was examined immunohistochemically in frozen sections. Four to 10 days following antigen challenge, 5 of the 20 animals showed significantly increased plasma cells in the subepithelial connective tissue of the endolymphatic sac (ES). Those cells showed positive reactions, mainly with IgG followed by IgM. The cells that reacted positively with IgA were few. Some of these plasma cells were considered to contain the specific antibody against HRP. The results indicate the role of the ES as a local immune response region for the inner ear complex, as well as the existence of an immunologic route from the middle ear cavity to the inner ear, particularly to the ES.
将20只豚鼠进行皮内注射辣根过氧化物酶（HRP）免疫，并在鼓泡内用5毫克相同抗原进行激发。通过免疫组织化学方法在冰冻切片中检查内耳结构中产生免疫球蛋白的细胞（浆细胞）的出现情况。抗原激发后4至10天，20只动物中有5只在内淋巴囊（ES）的上皮下结缔组织中显示出浆细胞显著增加。这些细胞显示出阳性反应，主要为IgG，其次为IgM。与IgA呈阳性反应的细胞很少。其中一些浆细胞被认为含有针对HRP的特异性抗体。结果表明ES作为内耳复合体局部免疫反应区域的作用，以及从中耳腔到内耳，特别是到ES的免疫途径的存在。
