Wang Rui, Shi Yan-ping, Wang Qing-zhong, Cao Hui
Zhongguo Zhong Yao Za Zhi. 2014 Dec;39(24):4811-5.
Column chromatography on silica gel was used to study the chemical constituents of traditional Chinese medicine Siegesbeckia pubescens. The chemical structures of the separated compounds were elucidated by spectroscopic data analyses. As a result, eighteen compounds were obtained and identified as 3, 4'-dimethoxy quercetin(1), 3, 3', 4'-trimethoxy quercetin(2), 3, 3'-dimethoxy quercetin(3), 7, 3', 4'-trimethoxy luteolin(4), ursolic acid(5), 2β,19α-dihydroxyursolic acid(6), 2β-hydroxyursolic acid (7), stigmasterol-7-one(8), 5α, 8α-epidioxy-24(R)-methyl-cholesta-6, 22-diene-3β-ol(9), β-sitosterol(10), 2, 6-di(3-hydroxy-4-methoxyphenyl)-3, 7-dioxacyclo [3. 3. 0] octane (11), aurantiamide acetate (12), 3-(m-hydroxyl-p-methoxy)-N-(2'-p-hydroxyl-phenethyl)-2E-acrylamide(13), p-hydroxy benzaldehyde (14), m-hydroxy-p-methoxy benzaldehyde (15), 3, 4, 5-trimethoxybenzoic acid(16), monoethyl malonate(17), and p-hydroxylcinnamic acid(18). Among them, compounds 1-9, 11-18 were isolated from this plant for the first time.
采用硅胶柱色谱法对中药毛梗稀莶的化学成分进行研究。通过光谱数据分析阐明分离得到化合物的化学结构。结果，共得到18个化合物，分别鉴定为3,4'-二甲氧基槲皮素（1）、3,3',4'-三甲氧基槲皮素（2）、3,3'-二甲氧基槲皮素（3）、7,3',4'-三甲氧基木犀草素（4）、熊果酸（5）、2β,19α-二羟基熊果酸（6）、2β-羟基熊果酸（7）、豆甾-7-酮（8）、5α,8α-环氧-24（R）-甲基-胆甾-6,22-二烯-3β-醇（9）、β-谷甾醇（10）、2,6-二（3-羟基-4-甲氧基苯基）-3,7-二氧杂环[3.3.0]辛烷（11）、橙黄酰胺乙酸酯（12）、3-（间羟基-对甲氧基）-N-（2'-对羟基苯乙基）-2E-丙烯酰胺（13）、对羟基苯甲醛（14）、间羟基-对甲氧基苯甲醛（15）、3,4,5-三甲氧基苯甲酸（16）、丙二酸单乙酯（17）、对羟基肉桂酸（18）。其中，化合物1-9、11-18为首次从该植物中分离得到。
