Jung Su-Young, Jung Won-Seok, Jung Ho-Kyung, Lee Gyeong-Hwan, Cho Jung-Hee, Cho Hyun-Woo, Choi In-Young
J Cosmet Sci. 2014 Nov-Dec;65(6):377-88.
Melanin is the pigment responsible for the color of the eyes, hair, and skin in humans. Tyrosinase is well known to be the key enzyme in melanin biosynthesis. JKTM-12 is composed of the flowers, roots, seeds, and receptacles of Nelumbo nucifera (lotus). In this study, JKTM-12 was investigated for its inhibitory effects on tyrosinase activity and melanin biosynthesis in B16F10 melanoma cells. Moreover, two main bioactive compounds (hyperoside and astragalin) were found from the receptacles of N. nucifera, which are used as the main material of JKTM-12. JKTM-12 was shown to inhibit tyrosinase activity and melanin biosynthesis in alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells. Hyperoside and astragalin, which are the main bioactive compounds of JKTM-12, not only inhibited tyrosinase activity and melanogenesis but also tyrosinase-related protein 1 and tyrosinase-related protein 2 mRNA expression without cytotoxicity at various experiment doses (0.1, 1, and 10 μg/ml). These results suggest that JKTM-12 has the potential for skin whitening with hyperoside and astragalin as the main bioactive compounds.
黑色素是决定人类眼睛、头发和皮肤颜色的色素。众所周知，酪氨酸酶是黑色素生物合成中的关键酶。JKTM - 12由莲（Nelumbo nucifera）的花、根、种子和花托组成。在本研究中，研究了JKTM - 12对B16F10黑色素瘤细胞中酪氨酸酶活性和黑色素生物合成的抑制作用。此外，从莲的花托中发现了两种主要的生物活性化合物（金丝桃苷和紫云英苷），它们被用作JKTM - 12的主要原料。结果表明，JKTM - 12在α - 黑素细胞刺激素刺激的B16F10黑色素瘤细胞中能抑制酪氨酸酶活性和黑色素生物合成。JKTM - 12的主要生物活性化合物金丝桃苷和紫云英苷，在不同实验剂量（0.1、1和10μg/ml）下不仅抑制酪氨酸酶活性和黑色素生成，还抑制酪氨酸酶相关蛋白1和酪氨酸酶相关蛋白2的mRNA表达，且无细胞毒性。这些结果表明，以金丝桃苷和紫云英苷作为主要生物活性化合物的JKTM - 12具有皮肤美白的潜力。
