Shimizu K, Kondo R, Sakai K, Lee S H, Sato H
Department of Forest Products, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Planta Med. 1998 Jun;64(5):408-12. doi: 10.1055/s-2006-957470.
The inhibitory effects of methanol extracts of heartwood of 23 Papua New Guinean wood species on tyrosinase activity were examined. The extract of Artocarpus incisus showed the strongest tyrosinase inhibitory activity which was equivalent to kojic acid. The extract apparently inhibited melanin biosynthesis of both cultured B16 melanoma cells without any cytotoxicity and in the back of a brown guinea pig without skin irritation. Thus, the potentiality of the extracts of heartwood of A. incisus both as material of a useful skin whitening agent and as a remedy for disturbances in pigmentation is evident. Tyrosinase inhibitory activity-guided fractionation led to the isolation of seven active compounds including a new compound which has been characterized as 6-(3"-methyl-1"-butenyl)-5,7,2',4'-tetrahydroxyflavone, named isoartocarpesin. Other active compounds were (+)-dihydromorin, chlorophorin, (+)-norartocarpanone, 4-prenyloxyresveratrol, artocarbene, and artocarpesin, These compounds are probably responsible for the melanin biosynthesis inhibitory effects.
研究了23种巴布亚新几内亚木材的心材甲醇提取物对酪氨酸酶活性的抑制作用。面包树的心材提取物表现出最强的酪氨酸酶抑制活性，与曲酸相当。该提取物明显抑制培养的B16黑色素瘤细胞的黑色素生物合成，且无任何细胞毒性，同时对棕色豚鼠背部皮肤无刺激。因此，面包树心材提取物作为有用的皮肤美白剂原料以及色素沉着紊乱治疗药物的潜力是显而易见的。通过酪氨酸酶抑制活性导向的分级分离，分离出了七种活性化合物，其中包括一种新化合物，该化合物已被鉴定为6-(3”-甲基-1”-丁烯基)-5,7,2',4'-四羟基黄酮，命名为异面包树素。其他活性化合物为(+)-二氢桑色素、绿叶胆色素、(+)-去甲面包树酮、4-异戊烯氧基白藜芦醇、面包树烯和面包树素，这些化合物可能是黑色素生物合成抑制作用的原因。
