Keely S P, Brinkman N E, Zimmerman B D, Wendell D, Ekeren K M, De Long S K, Sharvelle S, Garland J L
National Exposure Research Laboratory, United States Environmental Protection Agency, Cincinnati, OH, USA.
McMicken College of Arts and Sciences, Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA.
J Appl Microbiol. 2015 Jul;119(1):289-301. doi: 10.1111/jam.12835. Epub 2015 May 19.
Development of efficacious grey water (GW) treatment systems would benefit from detailed knowledge of the bacterial composition of GW. Thus, the aim of this study was to characterize the bacterial composition from (i) various points throughout a GW recycling system that collects shower and sink handwash (SH) water into an equalization tank (ET) prior to treatment and (ii) laundry (LA) water effluent of a commercial-scale washer.
Bacterial composition was analysed by high-throughput pyrosequencing of the 16S rRNA gene. LA was dominated by skin-associated bacteria, with Corynebacterium, Staphylococcus, Micrococcus, Propionibacterium and Lactobacillus collectively accounting for nearly 50% of the total sequences. SH contained a more evenly distributed community than LA, with some overlap (e.g. Propionibacterium), but also contained distinct genera common to wastewater infrastructure (e.g. Zoogloea). The ET contained many of these same wastewater infrastructure-associated bacteria, but was dominated by genera adapted for anaerobic conditions.
The data indicate that a relatively consistent set of skin-associated genera are the dominant human-associated bacteria in GW, but infrastructure-associated bacteria from the GW collection system and ET used for transient storage will be the most common bacteria entering GW treatment and reuse systems.
This study is the first to use high-throughput sequencing to identify the bacterial composition of various GW sources.
开发有效的中水（GW）处理系统将受益于对GW细菌组成的详细了解。因此，本研究的目的是对以下细菌组成进行表征：（i）整个GW回收系统中的各个点，该系统在处理前将淋浴和水槽洗手（SH）水收集到均衡池（ET）中；（ii）商业规模洗衣机的洗衣（LA）废水。
通过对16S rRNA基因进行高通量焦磷酸测序分析细菌组成。LA以与皮肤相关的细菌为主，棒状杆菌属、葡萄球菌属、微球菌属、丙酸杆菌属和乳杆菌属合计占总序列的近5０％。SH中的群落分布比LA更均匀，有一些重叠（如丙酸杆菌属），但也包含废水基础设施中常见的不同属（如动胶菌属）。ET含有许多这些与废水基础设施相关的相同细菌，但以适应厌氧条件的属为主。
数据表明，一组相对一致的与皮肤相关的属是GW中主要的与人类相关的细菌，但来自GW收集系统和用于临时储存的ET的与基础设施相关的细菌将是进入GW处理和再利用系统的最常见细菌。
本研究首次使用高通量测序来鉴定各种GW来源的细菌组成。
