Casas-Díaz Encarna, Closa-Sebastià Francesc, Marco Ignasi, Lavín Santiago, Bach-Raich Ester, Cuenca Rafaela
Servei d'Ecopatologia de Fauna Salvatge (SEFaS) - Wildlife Health Research Group, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Servei d'Hematologia Clínica Veterinària (SHCV) - Veterinary Clinical Hematology Service, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Vet Clin Pathol. 2015 Jun;44(2):215-22. doi: 10.1111/vcp.12250. Epub 2015 Apr 20.
Establishing reference intervals (RI) for hematologic and biochemical variables in wild animals presents great challenges because capture stress or anesthesia during sampling can affect blood variables.
The aims of this study were to establish RI for hematologic and blood biochemistry variables for Wild Boar (Sus scrofa) caught using cage traps, and provide information on the studied variables for different age groups.
Blood samples were obtained from 89 Wild Boars captured by cage trap between 2005 and 2013 in northeastern Spain. Piglets were handled without anesthesia, while juvenile and adult animals were anesthetized using a combination of tiletamine and zolazepam. Blood samples were collected from the anterior vena cava and were placed into plain and EDTA tubes. Thirteen hematologic and 21 biochemical variables were determined. Reference intervals for piglets and juvenile and adult groups were determined, and differences between these groups were statistically analyzed.
Adults had higher HGB, PCV, MCH, MCHC, neutrophil count, and total protein, albumin, creatinine, and chloride concentrations than juveniles; in contrast, juveniles had higher values for lymphocyte count, cholesterol concentration, and ALP activity.
Reference intervals determined in this study provide a baseline for interpreting hematologic and biochemical results in Wild Boar at different age stages, and contribute to optimization of the management of this species.
为野生动物的血液学和生化指标建立参考区间面临巨大挑战，因为采样过程中的捕捉应激或麻醉会影响血液指标。
本研究旨在为使用笼式陷阱捕获的野猪（Sus scrofa）建立血液学和血液生化指标的参考区间，并提供不同年龄组所研究指标的相关信息。
2005年至2013年期间，从西班牙东北部用笼式陷阱捕获的89头野猪采集血样。仔猪采样时未进行麻醉，而幼年和成年动物使用替来他明和唑拉西泮联合麻醉。从前腔静脉采集血样，并放入普通管和乙二胺四乙酸（EDTA）管中。测定了13项血液学指标和21项生化指标。确定了仔猪组以及幼年和成年组的参考区间，并对这些组之间的差异进行了统计学分析。
成年野猪的血红蛋白（HGB）、红细胞压积（PCV）、平均红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）、中性粒细胞计数以及总蛋白、白蛋白、肌酐和氯离子浓度均高于幼年野猪；相比之下，幼年野猪的淋巴细胞计数、胆固醇浓度和碱性磷酸酶（ALP）活性较高。
本研究确定的参考区间为解释不同年龄阶段野猪的血液学和生化结果提供了基线，并有助于优化该物种的管理。
