Dehghani Hossein, Hashemi Mehrdad, Entezari Maliheh, Mohsenifar Afshin
Department of Medical Laboratory Sciences, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran .
Department of Genetics, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran .
Iran J Pharm Res. 2015 Spring;14(2):539-46.
Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.
癌症是全球主要死因之一，由环境物理化学诱变剂和致癌剂作用所致。研发对免疫系统副作用小的新型细胞毒性药物已成为药理学新研究中的重要领域。源自药用香料黑种草（又称黑孜然）的百里醌（TQ）具有抗炎和抗癌活性。在本研究中，我们采用基于纳米凝胶的纳米颗粒方法来提高其有效性。通过自组装技术制备了肉豆蔻酸 - 壳聚糖（MA - 壳聚糖）纳米凝胶。将百里醌负载到纳米凝胶中。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）确定所制备纳米颗粒的表面形态。本研究的另一个目的是检测百里醌和纳米百里醌对人乳腺腺癌细胞系（MCF7）细胞死亡的体外细胞毒性活性。通过3 -（4,5 - 二甲基噻唑 - 2 - 基）-2,5 - 二苯基四氮唑溴盐（MTT）和染料排除试验评估百里醌和纳米百里醌的细胞毒性和活力。透射电子显微镜证实粒径在150至200nm之间。在特定时间内，百里醌和纳米百里醌以浓度依赖的方式显著抑制MCF7细胞的增殖。百里醌和纳米百里醌的半数抑制浓度（IC50）存在显著差异。与百里醌溶液相比，负载百里醌的纳米颗粒证明更有效。高药物靶向潜力和效率证明了负载百里醌的纳米颗粒抗癌特性的重要作用。
