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海滨锦葵幼苗响应盐胁迫的全基因组表达

Global Gene Expression of Kosteletzkya virginica Seedlings Responding to Salt Stress.

作者信息

Tang Xiaoli, Wang Hongyan, Shao Chuyang, Shao Hongbo

机构信息

Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, 264003, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, 264003, China; Yantai Academy of China Agriculture University, Yantai, 264670, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

出版信息

PLoS One. 2015 Apr 22;10(4):e0124421. doi: 10.1371/journal.pone.0124421. eCollection 2015.

Abstract

Soil salinization is becoming a serious threat to crop yield all over the world. Nowadays, acquainting the specific molecular mechanisms underlying various abiotic stresses especially to salt stress should be of great importance. While the development of the high-throughout sequencing technology promoted the progress powerfully. The intricate perception, transduction and regulation mechanisms underlying salt stress are being illustrated more and more clearly. As a perennial halophytic plant, Kosteletzkya virginica is able to help us to understand the mechanisms more directly and effectively. We carried out the whole transcriptome analysis on young seedlings with or without salt treatment through high-throughout sequencing technology. The results revealed that the numbers of different expressed transcripts between control and different treatments are 4145 and 9134, respectively. The ORF prediction suggested that there were 94308 ORF out of the 103489 (91.10%) total transcripts. We also carried out further differential expression analysis through gene ontology (GO) classification, cluster of orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In a word, our transcriptome study on Kosteletzkya virginica would provide direct and effective references for researches on molecular mechanisms of salt-tolerance, extending our view of salt tolerance in plant further. Above all, the related report in this paper is the first about Kosteletzkya virginica.

摘要

土壤盐渍化正成为全球农作物产量的严重威胁。如今,了解各种非生物胁迫尤其是盐胁迫背后的具体分子机制至关重要。高通量测序技术的发展有力地推动了这一进程。盐胁迫背后复杂的感知、转导和调控机制正越来越清晰地得到阐释。作为一种多年生盐生植物,弗吉尼亚科斯特莱特兹基草能够帮助我们更直接有效地理解这些机制。我们通过高通量测序技术对经盐处理和未经盐处理的幼苗进行了全转录组分析。结果显示,对照与不同处理之间差异表达转录本的数量分别为4145和9134。开放阅读框预测表明,在103489条(91.10%)总转录本中共有94308个开放阅读框。我们还通过基因本体(GO)分类、直系同源簇(COG)和京都基因与基因组百科全书(KEGG)分析进行了进一步的差异表达分析。总之,我们对弗吉尼亚科斯特莱特兹基草的转录组研究将为耐盐分子机制的研究提供直接有效的参考,进一步拓展我们对植物耐盐性的认识。最重要的是,本文的相关报道是关于弗吉尼亚科斯特莱特兹基草的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b386/4406580/ce3f5c7d252b/pone.0124421.g001.jpg

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