Kai Ng Shy, Nee Tee Ai, Ling Elaine Lai Chia, Ping Tan Chin, Kamariah Long, Lin Nyam Kar
Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia.
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Asian Pac J Trop Med. 2015 Jan;8(1):6-13. doi: 10.1016/S1995-7645(14)60179-6.
To determine the antihypercholesterolemic effects of kenaf seed samples and compare with the commercial hypocholesterolemic drug on serum lipids profiles and malondialdehyde (MDA) level in the rat.
Kenaf seed oil (KSO), microencapsulated kenaf seed oil (MKSO), kenaf seed extract (KSE) and defatted kenaf seed meal (DKSM) were prepared and phytochemicals screening on these samples were done prior in vivo study. Phenolic compounds in KSE were quantified using high performance liquid chromatography. There were 40 (divided in eight diet groups of 5) male Sprague dawley rats adapted to normal standard diet or hypercholesterolemic diet (HD) with or without the treatment of these kenaf samples for 32 days.
All the kenaf samples exhibited to contain most of the major phytochemicals. KSE possessed gallic acid, tannic acid, catechin, benzaldehyde, benzoic acid, syringic acid, sinapic acid, ferulic acid, naringin acid, and protocatechuic acid. The significant higher (P<0.05) serum total cholesterol, low density lipoprotein cholesterol and MDA levels in HD group without treatment than the normal control group suggested the hypercholesterolemia was induced by the incorporation of cholesterol into diet. KSE exhibited higher cholesterol-lowering properties due to the significant lower (P<0.05) in serum triglycerides, total cholesterol and MDA levels. KSE showed the highest efficiency of cholesterol-lowering activity, followed by KSO, MKSO and DKSM.
DKSM, MKSO, KSO and KSE appeared to have comparable anti-hypercholesterolemic effect with the commercial hypocholesterolemic drug. Hence, kenaf seed could be used as an alternative natural source to replace the synthetic hypocholesterolemic drugs.
确定洋麻籽样品的抗高胆固醇血症作用，并与市售降胆固醇药物比较对大鼠血脂谱和丙二醛（MDA）水平的影响。
制备洋麻籽油（KSO）、微胶囊化洋麻籽油（MKSO）、洋麻籽提取物（KSE）和脱脂洋麻籽粕（DKSM），并在体内研究前对这些样品进行植物化学筛选。使用高效液相色谱法对KSE中的酚类化合物进行定量。将40只雄性Sprague dawley大鼠（分为8个饮食组，每组5只）适应正常标准饮食或高胆固醇饮食（HD），并在有或无这些洋麻样品处理的情况下喂养32天。
所有洋麻样品均显示含有大多数主要植物化学物质。KSE含有没食子酸、单宁酸、儿茶素、苯甲醛、苯甲酸、丁香酸、芥子酸、阿魏酸、柚皮苷酸和原儿茶酸。未处理的HD组血清总胆固醇、低密度脂蛋白胆固醇和MDA水平显著高于（P<0.05）正常对照组，表明饮食中添加胆固醇可诱发高胆固醇血症。KSE由于血清甘油三酯、总胆固醇和MDA水平显著降低（P<0.05），表现出较高的降胆固醇特性。KSE显示出最高的降胆固醇活性效率，其次是KSO、MKSO和DKSM。
DKSM、MKSO、KSO和KSE似乎具有与市售降胆固醇药物相当的抗高胆固醇血症作用。因此，洋麻籽可作为替代天然来源，以取代合成降胆固醇药物。
