Wong Yu Hua, Tan Wai Yan, Tan Chin Ping, Long Kamariah, Nyam Kar Lin
Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia.
Department of Food Technology, Faculty of Food Science and Technology,Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Asian Pac J Trop Biomed. 2014 May;4(Suppl 1):S510-5. doi: 10.12980/APJTB.4.2014C1090.
To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines.
The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope.
The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected.
KSE and KSO could be potential sources of natural anti-cancer agents. Further investigations on using kenaf seeds for anti-proliferative properties are warranted.
研究洋麻（Hibiscus cannabinus L.）种子提取物和洋麻籽油对人子宫颈癌、人乳腺癌、人结肠癌和人肺癌细胞系的细胞毒性特性。
采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐和磺酰罗丹明B检测法，评估洋麻（Hibiscus cannabinus L.）种子提取物和洋麻籽油对人癌细胞系的体外细胞毒性活性。使用倒置光学显微镜观察细胞形态变化。
在所有癌细胞系中，洋麻种子提取物（KSE）的半数抑制浓度（IC50）低于洋麻籽油（KSO）。观察到KSE和KSO处理后细胞系的形态改变。KSE和KSO对所有选定的细胞系均具有有效的细胞毒性活性。
KSE和KSO可能是天然抗癌剂的潜在来源。有必要进一步研究洋麻种子的抗增殖特性。
