Liu Xiao-Xi, Shi Yan
Department of endocrinology, Affiliated Hospital of Liaoning University of TMC, Shenyang 110032, China.
Department of endocrinology, Affiliated Hospital of Liaoning University of TMC, Shenyang 110032, China.
Asian Pac J Trop Med. 2015 Feb;8(2):162-8. doi: 10.1016/S1995-7645(14)60309-6.
To investigate effects of herbal compound Yi Tang Kang on the spleen deficiency metabolic syndrome.
Forty male Wistar rats were randomly divided into two groups: the normal control group and the MS spleen deficiency syndrome group. The control group rats were fed with standard diet and water, while MS spleen deficiency syndrome group with high fat diet and low dose intraperitoneal injection of streptozocin, which swam to the endurance limit. After 12 weeks, the MS spleen deficiency syndrome group was randomly divided into two groups, with 13 rats in each group. Rats in model group were fed with high fat diet and continuouly administered with daily saline, and rats in intervention group with high fat diet were trated with traditional Chinese medicines Yi Tang Kang by gavage, 2 mL/200 g at the same time every day. 10 weeks later, the expression of serum proteomics was investigated through abdominal aortic puncture and separation of serum, using isotope labeling technique, high performance liquid chromatography and four bar-Orbitrap mass spectrometer.
After treatment with traditional Chinese medicine yitangkang, in the model group, important carboxylesterase and retinal guanylate cyclase 2 precursor were upregulated. As for intervention group, these indesxes were raised, but immunoglobulin IgG, carnitine acetyltransferase, tubulin beta -5, and Gan Lu sugar binding protein C were down-regulated. At the same time, some new biological active substances, such as protein tyrosine kinase, beta glucosidase were also found.
Traditional Chinese medicines Yi Tang Kang could regulate glucose and lipid metabolism in rats with spleen deficiency syndrome.
探讨中药复方益糖康对脾虚代谢综合征的影响。
将40只雄性Wistar大鼠随机分为两组：正常对照组和脾虚代谢综合征组。对照组大鼠给予标准饲料和饮水，脾虚代谢综合征组给予高脂饲料并腹腔注射低剂量链脲佐菌素，直至游泳至耐力极限。12周后，将脾虚代谢综合征组随机分为两组，每组13只。模型组大鼠给予高脂饲料并每日灌胃生理盐水，干预组大鼠给予高脂饲料并同时每日按2 mL/200 g的剂量灌胃中药益糖康。10周后，通过腹主动脉穿刺采集血清，采用同位素标记技术、高效液相色谱和四极杆-轨道阱质谱仪研究血清蛋白质组学表达。
益糖康治疗后，模型组中重要的羧酸酯酶和视网膜鸟苷酸环化酶2前体上调。干预组中，这些指标升高，但免疫球蛋白IgG、肉碱乙酰转移酶、微管蛋白β-5和甘露糖结合蛋白C下调。同时，还发现了一些新的生物活性物质，如蛋白酪氨酸激酶、β-葡萄糖苷酶。
中药益糖康可调节脾虚综合征大鼠的糖脂代谢。
