Li Wei, Yu Zhuling, Hou Deren, Zhou Lin, Deng Yanyao, Tian Mi, Feng Xialu
Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China.
Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China; Department of Nerve medical center, The First Hospital of Changsha, Changsha, China.
PLoS One. 2015 Apr 22;10(4):e0125186. doi: 10.1371/journal.pone.0125186. eCollection 2015.
In recent years, researchers have found that adiponectin (ANP) plays an important role in the pathogenesis of Alzheimer's disease (AD), and low serum concentrations of ANP are associated with AD. Higher plasma ANP level have a protective effect against the development of cognitive decline, suggesting that ANP may affect AD onset. Meanwhile, accumulating evidence supports the crucial role of ANP in the pathogenesis of AD. To study the relationship between ANP gene polymorphisms (rs266729, -11377C>G and rs1501299, G276T) and late-onset AD (LOAD), we carried out a case-control study that included 201 LOAD patients and 257 healthy control subjects. Statistically significant differences were detected in the genotype and allelotype frequency distributions of rs266729 and rs1501299 between the LOAD group and the control group, with a noticeable increase in the G and T allelotype frequency distributions in the LOAD group (P < 0.05). Logistic regression analysis using recessive model and additive model revealed that the rs266729 GG and rs1501299 TT genotypes are associated with a greater risk of LOAD. Haplotype analysis identified four haplotypes: CG, CT, GG, and GT. The frequencies of the CT and GG haplotypes were not significantly different (P > 0.05) between the LOAD group and control group, whereas the CG and GT haplotypes were significantly different (P < 0.05), suggesting a negative correlation between the CG haplotype and LOAD onset (OR = 0.74, 95% CI = 0.57-0.96, P = 0.022), and a positive correlation between the GT haplotype and LOAD onset (OR = 2.29, 95% CI = 1.42-3.68, P = 0.005). Therefore, we speculated that the rs266729 and rs1501299 of ANP gene polymorphisms and the GT and CG haplotypes were associated with LOAD.
近年来，研究人员发现脂联素（ANP）在阿尔茨海默病（AD）的发病机制中起重要作用，血清ANP浓度低与AD相关。较高的血浆ANP水平对认知功能下降的发展具有保护作用，提示ANP可能影响AD的发病。同时，越来越多的证据支持ANP在AD发病机制中的关键作用。为研究ANP基因多态性（rs266729、-11377C>G和rs1501299、G276T）与晚发型AD（LOAD）的关系，我们开展了一项病例对照研究，纳入201例LOAD患者和257例健康对照者。在LOAD组和对照组之间，rs266729和rs1501299的基因型和等位基因频率分布存在统计学显著差异，LOAD组中G和T等位基因频率分布明显增加（P<0.05）。使用隐性模型和加性模型的逻辑回归分析显示，rs266729 GG和rs1501299 TT基因型与LOAD风险增加相关。单倍型分析确定了四种单倍型：CG、CT、GG和GT。LOAD组和对照组之间CT和GG单倍型的频率无显著差异（P>0.05），而CG和GT单倍型有显著差异（P<0.05），提示CG单倍型与LOAD发病呈负相关（OR=0.74，95%CI=0.57-0.96，P=0.022），GT单倍型与LOAD发病呈正相关（OR=2.29，95%CI=1.42-3.68，P=0.005）。因此，我们推测ANP基因多态性的rs266729和rs1501299以及GT和CG单倍型与LOAD相关。
