核因子κB抑制剂PDTC对Lewis肺癌小鼠血管内皮生长因子和内皮抑素表达的影响

Effect of NF- κ B inhibitor PDTC on VEGF and endostatin expression of mice with Lewis lung cancer.

作者信息

Gao Ping, Gao Ya-Jie, Liang Hong-Lu

机构信息

Oncology Department, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116000, China.

出版信息

Asian Pac J Trop Med. 2015 Mar;8(3):220-4. doi: 10.1016/S1995-7645(14)60319-9.

DOI:10.1016/S1995-7645(14)60319-9

PMID:25902165

Abstract

OBJECTIVE

To investigate the effects of NF- κ B inhibitor pyrrolidine dithiocarbamate hydrochloride (PDTC) on vascular endothelial growth factor (VEGF) and endostatin expression in mice with Lewis lung cance; and its mechanism.

METHODS

Mice survival rate and anti-tumor effects were observed in different concentrations of NF- κ B inhibitor PDTC after the Lewis lung cancer mice model was established. VEGF and endostatin expressions were detected by immunohistochemical assay.

RESULTS

Lewis lung cancer was be inhibited by 0.5 mg/kg, 1.5 mg/kg and 3.0 mg/kg of NF- κ B inhibitor PDTC (P<0.05). Microvessel density (MVD) in 0.5 mg/kg, 1.5 mg/kg and 3.0 mg/kg NF- κ B inhibitor PDTC groups were significantly lower than the control group (P<0.05). Immunohistochemical assay results showed that VEGF and endostatin expressions in the 0.5 mg/kg, 1.5 mg/kg and 3.0 mg/kg NF-κ B inhibitor PDTC groups were significantly lower than the control group (P<0.05). Western blot results also showed that NF- κ B inhibitor PDTC could inhibit VEGF and endostatin expressions in tumor tissues.

CONCLUSIONS

NF- κ B inhibitor PDTC can inhibit tumor formation and reduce tumor angiogenesis in mice with Lewis lung cancer; and its mechanism maybe associated to VEGF and endostatin down-regulation.

摘要

目的

探讨核因子κB（NF-κB）抑制剂盐酸吡咯烷二硫代氨基甲酸盐（PDTC）对Lewis肺癌小鼠血管内皮生长因子（VEGF）和内皮抑素表达的影响及其机制。

方法

建立Lewis肺癌小鼠模型后，观察不同浓度的NF-κB抑制剂PDTC对小鼠生存率和抗肿瘤作用的影响。采用免疫组织化学法检测VEGF和内皮抑素的表达。

结果

0.5mg/kg、1.5mg/kg和3.0mg/kg的NF-κB抑制剂PDTC可抑制Lewis肺癌生长（P<0.05）。0.5mg/kg、1.5mg/kg和3.0mg/kg NF-κB抑制剂PDTC组的微血管密度（MVD）显著低于对照组（P<0.05）。免疫组织化学检测结果显示，0.5mg/kg、1.5mg/kg和3.0mg/kg NF-κB抑制剂PDTC组的VEGF和内皮抑素表达显著低于对照组（P<0.05）。蛋白质印迹法结果也显示，NF-κB抑制剂PDTC可抑制肿瘤组织中VEGF和内皮抑素的表达。