Chen Wei-Min, Zhang Xiao-Lei, Chai Hong-Mei, Chen Li-Jiao, Liu Wu-Long, Zhao Yong-Chang
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221, Yunnan, People's Republic of China.
Curr Microbiol. 2015 Aug;71(2):204-13. doi: 10.1007/s00284-015-0819-5. Epub 2015 Apr 24.
The large number of spores produced by edible mushrooms cause many problems, including causing lung disease, depleting natural genetic diversity, and reduced quality of fruiting bodies. Obtaining spore-deficient strains and understanding the underlying molecular mechanisms of such strains are important for breeding work. In this study, we crossed monokaryotic strains isolated from the edible fungi Agrocybe salicacola to obtain three spore-deficient strains with losses of the sterigmata on the surface of the lamella. A mating test revealed that recessive alleles distributed in some strains might control sterigmata development during the mitotic or meiotic phases. Transcriptome analysis revealed that the majority of the genes involved in DNA mismatch repair, base excision repair, and homologous recombination exhibited down-regulated expression patterns in the mutant fruiting bodies. Five genetic fragments, which were highly similar to the GTP-cyclohydrolase encoding gene, the DNA repair gene rad 8, and cell wall integrity and stress response component-encoding genes, were all expressed exclusively in the wild-type strains; these findings provide important information for the study of the spore development of edible fungi.
食用菌产生的大量孢子会引发诸多问题，包括导致肺部疾病、消耗自然遗传多样性以及降低子实体品质。获得孢子缺陷型菌株并了解此类菌株潜在的分子机制对于育种工作至关重要。在本研究中，我们将从食用菌柳叶田头菇分离得到的单核菌株进行杂交，获得了三个孢子缺陷型菌株，这些菌株的菌褶表面没有小梗。交配试验表明，分布于某些菌株中的隐性等位基因可能在有丝分裂或减数分裂阶段控制小梗的发育。转录组分析显示，参与DNA错配修复、碱基切除修复和同源重组的大多数基因在突变子实体中呈现下调表达模式。五个与GTP-环水解酶编码基因、DNA修复基因rad 8以及细胞壁完整性和应激反应成分编码基因高度相似的基因片段，均仅在野生型菌株中表达；这些发现为食用菌孢子发育的研究提供了重要信息。
