Chen Weimin, Chai Hongmei, Yang Weixian, Zhang Xiaolei, Chen Yuhui, Zhao YongChang
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, Yunnan, China.
Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650223, Yunnan, China.
Curr Microbiol. 2017 Jun;74(6):772-778. doi: 10.1007/s00284-017-1247-5. Epub 2017 Apr 10.
Agrocybe salicacola is a delicious and cultivable mushroom. It is important to understand this species' inherent characteristics, especially to elucidate the constitution and segregation of mating genes. In this study, two compatible B mating loci in strain YAASM0711 of A. salicacola were cloned from the monokaryons, and sequence and phylogeny analyses showed two conserved genes encoding pheromone receptors maybe lost mating activity, which determined by comparing with those of other mushrooms. In the conserved regions of mating loci, partial insertion/deletion fragments made non-coding regions posses polymorphisms, and monokaryotic strains of different mating types were distinguished from each other according to the amplification profile of variable regions, which suggested mating loci were integrally assigned to offspring strains during mitosis in A. salicacola. As our known, it is the first to develop molecular markers for B mating-type identification using variable non-coding fragments of mating loci in basidiomycetes.
柳生田头菇是一种美味且可栽培的蘑菇。了解该物种的固有特性非常重要，尤其是阐明交配基因的组成和分离情况。在本研究中，从单核体中克隆了柳生田头菇菌株YAASM0711中的两个兼容B交配位点，序列和系统发育分析表明，与其他蘑菇相比，两个编码信息素受体的保守基因可能失去了交配活性。在交配位点的保守区域，部分插入/缺失片段使非编码区域具有多态性，不同交配类型的单核菌株根据可变区域的扩增图谱相互区分，这表明在柳生田头菇的有丝分裂过程中，交配位点完整地分配给了后代菌株。据我们所知，这是首次利用担子菌交配位点的可变非编码片段开发用于B交配型鉴定的分子标记。
