Tamba M
Istituto F.R.A.E. del C.N.R., Bologna, Italy.
Z Naturforsch C J Biosci. 1989 Sep-Oct;44(9-10):857-62. doi: 10.1515/znc-1989-9-1027.
Using the technique of pulse radiolysis, it has been demonstrated that thiyl radicals (RS.) derived from glutathione (GSH), cysteine (CYSH), penicillamine (PnSH) and 2-mercaptoethanol (ME) interact with oxygen at a high rate. The resulting transient absorption, with a maximum around 540-560 nm, is characteristic of the sulphur peroxyl radical (RSOO.). The yield and the kinetic of formation of RSOO. further support our previous suggestion that thiyl/O2 reaction is an equilibrium. The redox properties of RSOO. are discussed on the basis of the interaction with reductants. Studies on the radio-induced enzyme inactivation in the presence of thiols seem to suggest a damaging role for RSOO. radicals.
运用脉冲辐解技术已证明，源自谷胱甘肽（GSH）、半胱氨酸（CYSH）、青霉胺（PnSH）和2-巯基乙醇（ME）的硫自由基（RS.）能以高速率与氧气相互作用。所产生的瞬态吸收在540 - 560 nm左右有最大值，这是过氧硫自由基（RSOO.）的特征。RSOO.的生成产率和动力学进一步支持了我们之前提出的硫自由基/氧气反应是一个平衡反应的观点。基于与还原剂的相互作用，对RSOO.的氧化还原性质进行了讨论。在存在硫醇的情况下对辐射诱导的酶失活的研究似乎表明RSOO.自由基具有破坏作用。
