Pomraning Kyle R, Wei Siwei, Karagiosis Sue A, Kim Young-Mo, Dohnalkova Alice C, Arey Bruce W, Bredeweg Erin L, Orr Galya, Metz Thomas O, Baker Scott E
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
Fundamental and Computer Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, United States of America.
PLoS One. 2015 Apr 23;10(4):e0123188. doi: 10.1371/journal.pone.0123188. eCollection 2015.
Yarrowia lipolytica is an oleaginous ascomycete yeast that accumulates large amounts of lipids and has potential as a biofuel producing organism. Despite a growing scientific literature focused on lipid production by Y. lipolytica, there remain significant knowledge gaps regarding the key biological processes involved. We applied a combination of metabolomic and lipidomic profiling approaches as well as microscopic techniques to identify and characterize the key pathways involved in de novo lipid accumulation from glucose in batch cultured, wild-type Y. lipolytica. We found that lipids accumulated rapidly and peaked at 48 hours during the five day experiment, concurrent with a shift in amino acid metabolism. We also report that exhaustion of extracellular sugars coincided with thickening of the cell wall, suggesting that genes involved in cell wall biogenesis may be a useful target for improving the efficiency of lipid producing yeast strains.
解脂耶氏酵母是一种产油子囊菌酵母，能积累大量脂质，具有作为生物燃料生产生物体的潜力。尽管关于解脂耶氏酵母脂质生产的科学文献越来越多，但在涉及的关键生物学过程方面仍存在重大知识空白。我们应用代谢组学和脂质组学分析方法以及显微镜技术，来识别和表征分批培养的野生型解脂耶氏酵母中从头合成脂质从葡萄糖积累的关键途径。我们发现，在为期五天的实验中，脂质迅速积累并在48小时达到峰值，同时氨基酸代谢发生转变。我们还报告说，细胞外糖耗尽与细胞壁增厚同时发生，这表明参与细胞壁生物合成的基因可能是提高脂质生产酵母菌株效率的有用靶点。
