Department of Biotechnology, Graduate School, Korea University, Seoul, 02841, South Korea.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
J Biotechnol. 2018 Apr 20;272-273:7-13. doi: 10.1016/j.jbiotec.2018.02.011. Epub 2018 Feb 27.
Lipid production by oleaginous Yarrowia lipolytica depends highly on culture environments, such as carbon sources, carbon/nitrogen (C/N) ratios, types of media, and cellular growth phases. In this study, the effects of media and carbon sources on lipid and metabolite production were investigated by profiling fatty acids and intracellular metabolites of Y. lipolytica grown in various media. The highest total fatty acid yield 114.04 ± 6.23 mg/g dry cell weight was achieved by Y. lipolytica grown in minimal medium with glycerol (SCG) in the exponential phase. The high lipid production by Y. lipolytica in SCG was presumed to be due to the higher C/N ratio in SCG than in the complex media. Moreover, glycerol promoted lipid production better than glucose in both complex and minimal media because glycerol can easily incorporate into the core of triglycerides. Metabolite profiling revealed that levels of long-chain fatty acids, such as stearic acid, palmitic acid, and arachidic acid, increased in SCG medium. Meanwhile, in complex media supplemented with either glucose or glycerol, levels of amino acids, such as cysteine, methionine, and glycine, highly increased. This metabolomic approach could be applied to modulate the global metabolic network of Y. lipolytica for producing lipids and other valuable products.
产油解脂耶氏酵母的油脂生产高度依赖于培养环境，如碳源、碳氮比（C/N）、培养基类型和细胞生长阶段。在这项研究中，通过分析在不同培养基中生长的解脂耶氏酵母的脂肪酸和细胞内代谢物，研究了培养基和碳源对油脂和代谢物生产的影响。在指数生长期，解脂耶氏酵母在含有甘油的最小培养基（SCG）中培养时，总脂肪酸产量最高，为 114.04±6.23mg/g 干细胞重量。SCG 中较高的 C/N 比可能是解脂耶氏酵母在 SCG 中产生高油脂的原因。此外，甘油在复杂培养基和最小培养基中的促进油脂生产的效果均优于葡萄糖，因为甘油可以很容易地掺入三酰基甘油的核心。代谢物分析表明，在 SCG 培养基中，长链脂肪酸（如硬脂酸、棕榈酸和花生酸）的水平增加。同时，在补充葡萄糖或甘油的复杂培养基中，半胱氨酸、蛋氨酸和甘氨酸等氨基酸的水平显著增加。这种代谢组学方法可用于调节解脂耶氏酵母的全局代谢网络，以生产油脂和其他有价值的产品。
