Liu Jie, Xia Bing, Ji Baocheng, Li Jingrong, Xiao Shiji, Ding Lisheng, Zhou Yan
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R.China.
Eur J Mass Spectrom (Chichester). 2015;21(1):37-44. doi: 10.1255/ejms.1317.
Probe electrospray ionization (PESI) is a recently developed ionization technique based on electrospray ionization (ESI) that generates electrospray from the tip of a solid needle. High tolerance to salts, requirements of a trace amount of sample and direct ambient sampling- are major advantages of PESI compared with conventional ESI. In this report, three pairs of isomeric lignans bearing tetra-hydrofuran with variable conformations from Gymnotheca chinensis were investigated by probe electrospray tandem mass spectrometry (PESI-MS/MS) in the positive ion mode. The diagnostic characteristics of these compounds were obtained and the isomers could be successfully distinguished by comparison with their breakdown curves, even though the isomers differed only in the conformation of some groups of the isomer pairs. This report provides a rapid and reliable method for the identification of trace amounts of isomeric lignans by PESI-MS/MS. Furthermore, application of PESI and breakdown curves should have value in mass spectrometry studies of isomeric natural products compounds.
探针电喷雾电离（PESI）是一种基于电喷雾电离（ESI）的最新开发的电离技术，它从固体针的尖端产生电喷雾。与传统的ESI相比，PESI对盐的高耐受性、对痕量样品的要求以及直接的常压采样是其主要优点。在本报告中，采用正离子模式的探针电喷雾串联质谱（PESI-MS/MS）对来自中国裸蒴的三对具有不同构象的含四氢呋喃的异构木脂素进行了研究。获得了这些化合物的诊断特征，即使异构体仅在异构体对的某些基团的构象上有所不同，通过与其裂解曲线进行比较也能够成功区分这些异构体。本报告提供了一种通过PESI-MS/MS快速可靠地鉴定痕量异构木脂素的方法。此外，PESI和裂解曲线的应用在异构天然产物化合物的质谱研究中应具有价值。
