Clean Energy Research Center, University of Yamanashi, Kofu, Japan.
Rapid Commun Mass Spectrom. 2010 Jun 15;24(11):1507-13. doi: 10.1002/rcm.4556.
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.
探针电喷雾电离（PESI）是电喷雾电离（ESI）的改良版本，其中用于采样和喷雾的毛细管被固体针代替。与传统的 ESI 相比，PESI 对盐的高容忍度和直接环境采样是其主要优点。在这项研究中，PESI-MS 用于实时监测一些生物和化学反应，如酸诱导的蛋白质变性、肽的氢/氘交换（HDX）和席夫碱形成。通过使用 PESI-MS，可以可重复性地获得时间分辨质谱和离子色谱图。实时 PESI-MS 监测可以直接提供参与反应的每种化学物质的详细信息，这对于更好地理解整个反应过程和优化反应参数非常有价值。由于其仪器设置简单、采样直接、样品制备和样品消耗少，PESI-MS 可以被认为是实时反应监测的潜在工具。
