Goldeck David, Pawelec Graham, Norman Kristina, Steinhagen-Thiessen Elisabeth, Oettinger Lilly, Haehnel Karin, Demuth Ilja
Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Tübingen, Germany.
School of Science and Technology, College of Arts and Science, Nottingham Trent University, Nottingham, UK.
Biogerontology. 2016 Feb;17(1):189-98. doi: 10.1007/s10522-015-9577-9. Epub 2015 Apr 24.
Hand-grip strength is strongly correlated with measures of muscle mass and can be taken to predict morbidity and mortality. The aim of this study was to investigate the relationship between hand-grip strength and other markers associated with immune ageing, such as Cytomegalovirus (CMV) infection, leukocyte telomere length and serum levels of inflammatory and anti-inflammatory markers in the elderly. We have assessed grip strength with the Smedley Dynamometer in younger (22-37 years) and older (60-85 years) men and women in a sample of people living in Berlin (the BASE-II study). Serum cytokine levels were determined by flow-cytometry, CMV serostatus via ELISA and leukocyte telomere length by quantitative PCR. IL-1β levels tended to be negatively associated with grip strength, but we did not find a significant association with IL-6 levels. CMV-seropositivity was not associated with higher levels of IL-1β, IL-6 or TNF, nor with weaker grip strength in men or women at any age. A putative general measure of organismal ageing, overall leukocyte telomere length, was also found not to be associated with lower grip strength in the elderly. Hand-grip strength remains an important biomarker independent of CMV infection or shorter telomere lengths, and poorly reflected in peripheral pro-inflammatory cytokine levels, all of which have been associated in some studies with frailty and mortality.
握力与肌肉量的测量指标密切相关，可用于预测发病率和死亡率。本研究的目的是调查握力与其他与免疫衰老相关的标志物之间的关系，如巨细胞病毒（CMV）感染、白细胞端粒长度以及老年人炎症和抗炎标志物的血清水平。我们在柏林的一个样本（BASE-II研究）中，使用斯梅德利测力计评估了年轻（22 - 37岁）和年长（60 - 85岁）男性和女性的握力。通过流式细胞术测定血清细胞因子水平，通过酶联免疫吸附测定法检测CMV血清状态，通过定量聚合酶链反应检测白细胞端粒长度。IL-1β水平倾向于与握力呈负相关，但我们未发现与IL-6水平存在显著关联。CMV血清阳性与IL-1β、IL-6或TNF的较高水平无关，也与任何年龄的男性或女性较弱的握力无关。一种假定的机体衰老综合测量指标，即总体白细胞端粒长度，也未发现与老年人较低的握力有关。握力仍然是一个重要的生物标志物，独立于CMV感染或较短的端粒长度，且在外周促炎细胞因子水平中反映不佳，而在一些研究中，所有这些都与虚弱和死亡率有关。
