§Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
ACS Appl Mater Interfaces. 2015 May 20;7(19):10511-7. doi: 10.1021/acsami.5b02008. Epub 2015 May 7.
We report an antibacterial surface that kills airborne bacteria on contact upon minutes of solar near-infrared (NIR) irradiation. This antibacterial surface employs reduced graphene oxide (rGO), a well-known near-infrared photothermal conversion agent, as the photosensitizer and is prepared by assembling oppositely charged polyelectrolyte-stabilized rGO sheets (PEL-rGO) on a quartz substrate with the layer-by-layer (LBL) technique. Upon solar irradiation, the resulting PEL-rGO LBL multilayer efficiently generates rapid localized heating and, within minutes, kills >90% airborne bacteria, including antibiotic-tolerant persisters, on contact, likely by permeabilizing their cellular membranes. The observed activity is retained even when the PEL-rGO LBL multilayer is placed underneath a piece of 3 mm thick pork tissue, indicating that solar light in the near-infrared region plays dominant roles in the observed activity. This work may pave the way toward NIR-light-activated antibacterial surfaces, and our PEL-rGO LBL multilayer may be a novel surface coating material for conveniently disinfecting biomedical implants and common objects touched by people in daily life in the looming postantibiotic era with only minutes of solar exposure.
我们报告了一种抗菌表面，它在接触到几分钟的太阳近红外（NIR）照射后，能立即杀死空气中的细菌。这种抗菌表面采用还原氧化石墨烯（rGO）作为光热转换剂，rGO 是一种众所周知的近红外光热剂，它被组装在带相反电荷的聚电解质稳定的 rGO 片（PEL-rGO）上，然后通过层层（LBL）技术将其固定在石英基底上。在太阳照射下，所得到的 PEL-rGO LBL 多层膜有效地产生快速局部加热，在几分钟内，通过渗透其细胞膜，能杀死接触到的>90%的空气中的细菌，包括抗生素耐药的持久菌。即使将 PEL-rGO LBL 多层膜放在 3 毫米厚的猪肉组织下面，也能保持观察到的活性，这表明近红外区域的太阳光是观察到的活性的主要作用。这项工作可能为近红外光激活的抗菌表面铺平道路，我们的 PEL-rGO LBL 多层膜可能是一种新型的表面涂层材料，用于在抗生素时代后方便地对生物医学植入物和人们日常生活中经常接触的普通物体进行消毒，只需要几分钟的太阳照射。
