线粒体DNA D环区体细胞突变对口腔鳞状细胞癌患者生存的影响。
Impact of somatic mutations in the D-loop of mitochondrial DNA on the survival of oral squamous cell carcinoma patients.
作者信息
Lin Jin-Ching, Wang Chen-Chi, Jiang Rong-San, Wang Wen-Yi, Liu Shih-An
机构信息
Department of Radiation Oncology, Taichung Veterans General Hospital, Taichung, Taiwan; Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Department of Otolaryngology, Taichung Veterans General Hospital, Taichung, Taiwan; Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
出版信息
PLoS One. 2015 Apr 23;10(4):e0124322. doi: 10.1371/journal.pone.0124322. eCollection 2015.
OBJECTIVES
The aim of this study was to investigate somatic mutations in the D-loop of mitochondrial DNA (mtDNA) and their impact on survival in oral squamous cell carcinoma patients.
MATERIALS AND METHODS
Surgical specimen confirmed by pathological examination and corresponding non-cancerous tissues were collected from 120 oral squamous cell carcinoma patients. The sequence in the D-loop of mtDNA from non-cancerous tissues was compared with that from paired cancer samples and any sequence differences were recognized as somatic mutations.
RESULTS
Somatic mutations in the D-loop of mtDNA were identified in 75 (62.5%) oral squamous cell carcinoma patients and most of them occurred in the poly-C tract. Although there were no significant differences in demographic and tumor-related features between participants with and without somatic mutation, the mutation group had a better survival rate (5 year disease-specific survival rate: 64.0% vs. 43.0%, P = 0.0266).
CONCLUSION
Somatic mutation in D-loop of mtDNA was associated with a better survival in oral squamous cell carcinoma patients.
目的
本研究旨在调查线粒体DNA（mtDNA）D环中的体细胞突变及其对口腔鳞状细胞癌患者生存的影响。
材料与方法
从120例口腔鳞状细胞癌患者中收集经病理检查确诊的手术标本及相应的癌旁组织。将癌旁组织mtDNA D环中的序列与配对癌样本的序列进行比较，任何序列差异均被视为体细胞突变。
结果
75例（62.5%）口腔鳞状细胞癌患者中鉴定出线粒体DNA D环中的体细胞突变，其中大部分发生在多聚C序列中。尽管有体细胞突变和无体细胞突变的参与者在人口统计学和肿瘤相关特征方面无显著差异，但突变组的生存率更高（5年疾病特异性生存率：64.0%对43.0%，P = 0.0266）。
结论
线粒体DNA D环中的体细胞突变与口腔鳞状细胞癌患者较好的生存率相关。
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