Roth M R, Avery R B, Welti R
Division of Biology, Kansas State University, Manhattan 66506.
Biochim Biophys Acta. 1989 Nov 27;986(2):217-24. doi: 10.1016/0005-2736(89)90470-7.
Dimethylsuberimidate was reacted with aqueous dispersions of dipalmitoylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, and dielaidoylphosphatidylethanolamine at pH 10 and at pH 8. The amount of amidine dimer formation was about four times greater above the gel-to-fluid phase transition of each lipid than below the transition. The transition temperature of each phosphatidylethanolamine, measured by steady-state fluorescence anisotropy of cis-parinaric acid, was lower at pH 10 than at pH 8 or in water. The ability of dimethylsuberimidate to discriminate between phosphatidylethanolamines in the fluid and gel phases should allow use of this reagent to identify phosphatidylethanolamine species within the gel or fluid lipid phase.
在pH值为10和pH值为8的条件下，将亚辛二酸二甲酯与二棕榈酰磷脂酰乙醇胺、二肉豆蔻酰磷脂酰乙醇胺、二月桂酰磷脂酰乙醇胺和二油酰磷脂酰乙醇胺的水分散体进行反应。在每种脂质的凝胶-流体相转变温度以上，脒二聚体的形成量比转变温度以下大约大四倍。通过顺式-十八碳四烯酸的稳态荧光各向异性测量，每种磷脂酰乙醇胺在pH值为10时的转变温度低于pH值为8时或在水中的转变温度。亚辛二酸二甲酯区分流体相和凝胶相中磷脂酰乙醇胺的能力，应允许使用该试剂来识别凝胶或流体脂质相中的磷脂酰乙醇胺种类。
