Roth M R, Smardo F L, Welti R
Division of Biology, Kansas State University, Manhattan 66506.
Chem Phys Lipids. 1989 Jul;51(1):39-46. doi: 10.1016/0009-3084(89)90064-9.
Phosphatidylethanolamines of various fatty acyl species in vortexed lipid dispersions were reacted with dimethylsuberimidate to produce dimeric products. The yield was 34% at pH 10 and 2% at pH 7. The crosslinked phosphatidylethanolamine species were separated from minor products and the reactants by extraction and two-dimensional thin-layer chromatography on silica gel G, gel filtration on lipophilic Sephadex, or C18-reversed phase high-performance liquid chromatography (HPLC). Reversed phase HPLC was also used to resolve the dimers into individual molecular species. Analysis of the dimers revealed the extinction coefficients at 205 nm to be higher than those of the reactant phosphatidylethanolamines. Proton nuclear magnetic resonance spectroscopy and chemical analysis implied that the dimers contain an amidine group.
在涡旋的脂质分散体中，各种脂肪酰基种类的磷脂酰乙醇胺与辛二酸二甲酯反应生成二聚体产物。在pH 10时产率为34%，在pH 7时为2%。通过在硅胶G上的萃取和二维薄层色谱、在亲脂性葡聚糖凝胶上的凝胶过滤或C18反相高效液相色谱（HPLC），将交联的磷脂酰乙醇胺种类与次要产物和反应物分离。反相HPLC还用于将二聚体分离成单个分子种类。对二聚体的分析表明，其在205 nm处的消光系数高于反应物磷脂酰乙醇胺的消光系数。质子核磁共振光谱和化学分析表明二聚体含有脒基。
