通过多频相荧光法研究Ca2+与磷脂双层的相互作用。
Ca2+ interaction with phospholipid bilayers studied by multifrequency phase fluorometry.
作者信息
Zolese G, Curatola G
机构信息
Institute of Biochemistry, Medical School, Ancona, Italy.
出版信息
Biosci Rep. 1989 Aug;9(4):497-502. doi: 10.1007/BF01117053.
PMID:2590725
Abstract
Calcium interaction with phospholipid membranes containing phosphatidic acid is studied by multifrequency phase fluorometry, using DPH as fluorescent molecule. DPH decay is analysed by a continuous distribution of lifetimes. The results suggest an increase of membrane heterogeneity at low calcium concentrations, without changes in the polarity of the environment surrounding the probe.
摘要
利用多频相荧光法，以DPH作为荧光分子，研究了钙与含磷脂酸的磷脂膜之间的相互作用。通过寿命的连续分布来分析DPH的衰减。结果表明，在低钙浓度下膜的异质性增加，而探针周围环境的极性没有变化。
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