Czujkowska A, Arciszewski M B
Municipal Zoological Garden in Warsaw, Ratuszowa 1/3, 03-461, Warsaw, Poland.
Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033, Lublin, Poland.
Anat Histol Embryol. 2016 Apr;45(2):115-23. doi: 10.1111/ahe.12179. Epub 2015 Apr 23.
Galanin is a neuropeptide widely present in the enteric nervous system of numerous animal species and exhibiting neurotransmittery/neuromodulatory roles. Colocalization patterns of galanin with substance P (SP), corticotropin-releasing factor (CRF) and calbindin were studied in the small intestine of the wild boar using immunofluorescence technique. We demonstrated the presence of SP in substantial populations of galanin-immunoreactive (IR) submucous neurons. Additionally, different amounts of nerve fibres exhibiting simultaneous presence of galanin and SP were noted in the small intestinal smooth musculature, submucous ganglia, lamina muscularis mucosae and mucosa. In the wild boar duodenum, jejunum and ileum, the co-expression of galanin and calbindin was limited to minor populations of submucous neurons only. Single galanin-/CRF-IR nerve fibres were exclusively present in the duodenal and jejunal (but not ileal) mucosa. These results strongly suggest that galanin participates in neuronal control of the wild boar small intestine also by functional co-operation with other biologically active neuropeptides.
甘丙肽是一种神经肽，广泛存在于多种动物物种的肠神经系统中，并发挥神经传递/神经调节作用。采用免疫荧光技术，研究了野猪小肠中甘丙肽与P物质（SP）、促肾上腺皮质激素释放因子（CRF）和钙结合蛋白的共定位模式。我们证实在大量甘丙肽免疫反应性（IR）黏膜下神经元中存在SP。此外，在小肠平滑肌、黏膜下神经节、黏膜肌层和黏膜中，发现了不同数量同时存在甘丙肽和SP的神经纤维。在野猪十二指肠、空肠和回肠中，甘丙肽和钙结合蛋白的共表达仅限于少数黏膜下神经元。仅在十二指肠和空肠（而非回肠）黏膜中存在单个甘丙肽/CRF免疫反应性神经纤维。这些结果强烈表明，甘丙肽还通过与其他生物活性神经肽的功能协作参与野猪小肠的神经控制。
